High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions

S HANDAYANI, D CHIU, E TJITRA, J KUO, D LAMPAH, Enny Kenangalem, L RENIA, G SNOUNOU, Ric Price, Nicholas Anstey, Bruce Russell

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Maturation of Plasmodium falciparum decreases the deformability of infected red blood cells (RBCs), increasing their clearance as they attempt to pass through endothelial slits of the splenic sinus. Previous studies of Plasmodium vivax-infected RBCs led to opposite conclusions with respect to cellular deformability. To resolve this controversy, P. vivax-infected RBCs were passed through a 2-?m microfluidic channel. In contrast to P. falciparum-infected RBCs, mature P. vivax-infected RBCs readily became deformed through 2-?m constrictions. After this extreme deformation, 67% of P. vivax-infected RBCs recovered a normal appearance; however, 15% of uninfected RBCs were destroyed. Results suggest mechanisms for both avoidance of splenic clearance and anemia in vivax malaria. � 2008 by the Infectious Diseases Society of America. All rights reserved.
    Original languageEnglish
    Pages (from-to)445-450
    Number of pages6
    JournalJournal of Infectious Diseases
    Volume199
    Issue number3
    Publication statusPublished - 2009

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    Plasmodium vivax
    Microfluidics
    Erythrocytes
    Plasmodium falciparum
    Hypersplenism
    Vivax Malaria
    Constriction

    Cite this

    HANDAYANI, S., CHIU, D., TJITRA, E., KUO, J., LAMPAH, D., Kenangalem, E., ... Russell, B. (2009). High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions. Journal of Infectious Diseases, 199(3), 445-450.
    HANDAYANI, S ; CHIU, D ; TJITRA, E ; KUO, J ; LAMPAH, D ; Kenangalem, Enny ; RENIA, L ; SNOUNOU, G ; Price, Ric ; Anstey, Nicholas ; Russell, Bruce. / High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions. In: Journal of Infectious Diseases. 2009 ; Vol. 199, No. 3. pp. 445-450.
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    title = "High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions",
    abstract = "Maturation of Plasmodium falciparum decreases the deformability of infected red blood cells (RBCs), increasing their clearance as they attempt to pass through endothelial slits of the splenic sinus. Previous studies of Plasmodium vivax-infected RBCs led to opposite conclusions with respect to cellular deformability. To resolve this controversy, P. vivax-infected RBCs were passed through a 2-?m microfluidic channel. In contrast to P. falciparum-infected RBCs, mature P. vivax-infected RBCs readily became deformed through 2-?m constrictions. After this extreme deformation, 67{\%} of P. vivax-infected RBCs recovered a normal appearance; however, 15{\%} of uninfected RBCs were destroyed. Results suggest mechanisms for both avoidance of splenic clearance and anemia in vivax malaria. � 2008 by the Infectious Diseases Society of America. All rights reserved.",
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    HANDAYANI, S, CHIU, D, TJITRA, E, KUO, J, LAMPAH, D, Kenangalem, E, RENIA, L, SNOUNOU, G, Price, R, Anstey, N & Russell, B 2009, 'High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions', Journal of Infectious Diseases, vol. 199, no. 3, pp. 445-450.

    High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions. / HANDAYANI, S; CHIU, D; TJITRA, E; KUO, J; LAMPAH, D; Kenangalem, Enny; RENIA, L; SNOUNOU, G; Price, Ric; Anstey, Nicholas; Russell, Bruce.

    In: Journal of Infectious Diseases, Vol. 199, No. 3, 2009, p. 445-450.

    Research output: Contribution to journalArticleResearchpeer-review

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    AU - TJITRA, E

    AU - KUO, J

    AU - LAMPAH, D

    AU - Kenangalem, Enny

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    AU - SNOUNOU, G

    AU - Price, Ric

    AU - Anstey, Nicholas

    AU - Russell, Bruce

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    AB - Maturation of Plasmodium falciparum decreases the deformability of infected red blood cells (RBCs), increasing their clearance as they attempt to pass through endothelial slits of the splenic sinus. Previous studies of Plasmodium vivax-infected RBCs led to opposite conclusions with respect to cellular deformability. To resolve this controversy, P. vivax-infected RBCs were passed through a 2-?m microfluidic channel. In contrast to P. falciparum-infected RBCs, mature P. vivax-infected RBCs readily became deformed through 2-?m constrictions. After this extreme deformation, 67% of P. vivax-infected RBCs recovered a normal appearance; however, 15% of uninfected RBCs were destroyed. Results suggest mechanisms for both avoidance of splenic clearance and anemia in vivax malaria. � 2008 by the Infectious Diseases Society of America. All rights reserved.

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    HANDAYANI S, CHIU D, TJITRA E, KUO J, LAMPAH D, Kenangalem E et al. High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions. Journal of Infectious Diseases. 2009;199(3):445-450.