A hydration procedure for frogs is described that involves two episodes of bladder emptying and rehydration prior to determining hydrated body-fluid osmolality values. The mean osmolality of hydrated Cyclorana species was similar for C. australis (lymph 225 mOsm/kg, urine 31 mOsm/kg), C. longipes (lymph 211 mOsm/kg, urine 40 mOsm/kg), C. cultripes (lymph 213 mOsm/kg, urine 14 mOsm/kg)and C. platycephala (lymph 214 mOsm/kg, urine 21 mOsm/kg). These standardised values provide a baseline for comparison with dehydrated and active frogs. Using this method the investigator can accurately determine standard mass (the mass of a hydrated frog with an empty bladder), which requires two or more weight measurements.
|Number of pages||4|
|Journal||Journal of the Royal Society of Western Australia|
|Publication status||Published - 2012|