Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases

Implication as a potential major allergen in crusted scabies

Annette Marie Dougall, Deborah Holt, Katja Fischer, Bart Currie, David J Kemp, Shelley Faye Walton

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)977-984
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume73
Issue number5
Publication statusPublished - 2005

Fingerprint

Sarcoptes scabiei
Dermatophagoides pteronyssinus
Scabies
Glutathione Transferase
Allergens
Northern Territory
Queensland
Mites
Immunoglobulin E
Communicable Diseases
Emerging Communicable Diseases
Pyroglyphidae
Parasitic Diseases
School Health Services
Allergy and Immunology
Serum
Recombinant Proteins
Biomedical Research
Hypersensitivity
Immunoglobulin G

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@article{d25580f40395471982adb316a126ba6f,
title = "Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: Implication as a potential major allergen in crusted scabies",
abstract = "Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.",
keywords = "allergen, glutathione transferase, glutathione transferase delta, glutathione transferase mu, immunoglobulin E, immunoglobulin G4, recombinant protein, unclassified drug, antibody specificity, antigen antibody reaction, article, controlled study, Dermatophagoides pteronyssinus, disease severity, gene expression system, human, hypersensitivity, immune response, immunoglobulin blood level, mite, molecular cloning, nonhuman, nucleotide sequence, parasitic skin disease, pathophysiology, prokaryote, Sarcoptes scabiei, scabies, sequence homology, Allergens, Animals, Escherichia coli, Glutathione Transferase, Humans, Hypersensitivity, Immediate, Immunoglobulin E, Immunoglobulin G, Molecular Sequence Data, Recombinant Proteins, Scabies, Sequence Analysis, DNA, Acari, Astigmata, Prokaryota, Psoroptes cervinus, Pyroglyphidae",
author = "Dougall, {Annette Marie} and Deborah Holt and Katja Fischer and Bart Currie and Kemp, {David J} and Walton, {Shelley Faye}",
year = "2005",
language = "English",
volume = "73",
pages = "977--984",
journal = "The American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "5",

}

Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases : Implication as a potential major allergen in crusted scabies. / Dougall, Annette Marie; Holt, Deborah; Fischer, Katja; Currie, Bart; Kemp, David J; Walton, Shelley Faye.

In: American Journal of Tropical Medicine and Hygiene, Vol. 73, No. 5, 2005, p. 977-984.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases

T2 - Implication as a potential major allergen in crusted scabies

AU - Dougall, Annette Marie

AU - Holt, Deborah

AU - Fischer, Katja

AU - Currie, Bart

AU - Kemp, David J

AU - Walton, Shelley Faye

PY - 2005

Y1 - 2005

N2 - Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.

AB - Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.

KW - allergen

KW - glutathione transferase

KW - glutathione transferase delta

KW - glutathione transferase mu

KW - immunoglobulin E

KW - immunoglobulin G4

KW - recombinant protein

KW - unclassified drug

KW - antibody specificity

KW - antigen antibody reaction

KW - article

KW - controlled study

KW - Dermatophagoides pteronyssinus

KW - disease severity

KW - gene expression system

KW - human

KW - hypersensitivity

KW - immune response

KW - immunoglobulin blood level

KW - mite

KW - molecular cloning

KW - nonhuman

KW - nucleotide sequence

KW - parasitic skin disease

KW - pathophysiology

KW - prokaryote

KW - Sarcoptes scabiei

KW - scabies

KW - sequence homology

KW - Allergens

KW - Animals

KW - Escherichia coli

KW - Glutathione Transferase

KW - Humans

KW - Hypersensitivity, Immediate

KW - Immunoglobulin E

KW - Immunoglobulin G

KW - Molecular Sequence Data

KW - Recombinant Proteins

KW - Scabies

KW - Sequence Analysis, DNA

KW - Acari

KW - Astigmata

KW - Prokaryota

KW - Psoroptes cervinus

KW - Pyroglyphidae

M3 - Article

VL - 73

SP - 977

EP - 984

JO - The American Journal of Tropical Medicine and Hygiene

JF - The American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 5

ER -