TY - JOUR
T1 - Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases
T2 - Implication as a potential major allergen in crusted scabies
AU - Dougall, Annette Marie
AU - Holt, Deborah
AU - Fischer, Katja
AU - Currie, Bart
AU - Kemp, David J
AU - Walton, Shelley Faye
PY - 2005
Y1 - 2005
N2 - Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
AB - Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia; Institute of Advanced Studies, Charles Darwin University, Darwin, Northern Territory, Australia; Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia; The Australian Centre for International and Tropical Health and Nutrition, The University of Queensland, Brisbane, Queensland, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione 5-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
KW - allergen
KW - glutathione transferase
KW - glutathione transferase delta
KW - glutathione transferase mu
KW - immunoglobulin E
KW - immunoglobulin G4
KW - recombinant protein
KW - unclassified drug
KW - antibody specificity
KW - antigen antibody reaction
KW - article
KW - controlled study
KW - Dermatophagoides pteronyssinus
KW - disease severity
KW - gene expression system
KW - human
KW - hypersensitivity
KW - immune response
KW - immunoglobulin blood level
KW - mite
KW - molecular cloning
KW - nonhuman
KW - nucleotide sequence
KW - parasitic skin disease
KW - pathophysiology
KW - prokaryote
KW - Sarcoptes scabiei
KW - scabies
KW - sequence homology
KW - Allergens
KW - Animals
KW - Escherichia coli
KW - Glutathione Transferase
KW - Humans
KW - Hypersensitivity, Immediate
KW - Immunoglobulin E
KW - Immunoglobulin G
KW - Molecular Sequence Data
KW - Recombinant Proteins
KW - Scabies
KW - Sequence Analysis, DNA
KW - Acari
KW - Astigmata
KW - Prokaryota
KW - Psoroptes cervinus
KW - Pyroglyphidae
M3 - Article
VL - 73
SP - 977
EP - 984
JO - The American Journal of Tropical Medicine and Hygiene
JF - The American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 5
ER -