@article{eac4f72f9ebe47d88343e6b39a78f353,
title = "Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var. hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model",
abstract = "Sarcoptes scabiei ({"}itch mite{"}) causes scabies, a disease of considerable human and veterinary significance. Little work has been done at the molecular level because of the difficulty of obtaining mites. We have used mites in skin from the bedding of crusted scabies patients for the construction of a library of 105 cDNAs from S. scabiei var. hominis cloned in the vector pGEX4T-2. We describe the isolation by immunoscreening of 2 clones, one of which (Ssag1) is homologous to and cross-reactive with the house dust mite Euroglyphus maynei allergen M-177, an apolipoprotein from hemolymph. Immunohistochemistry revealed that it is located around the internal organs and cuticle of the mite and in eggs. Although it was not found to be protective in a challenge trial, the rabbits did not exhibit typical crust characteristics. This work shows that it is now possible to conduct such challenge trials with cloned scabies antigens.",
keywords = "apolipoprotein, apolipoprotein m177, complementary DNA, house dust allergen, unclassified drug, vaccine, allergen, antigen, antiserum, messenger RNA, animal experiment, article, cross reaction, cuticle, Dermatophagoides, egg, gene isolation, hemolymph, immunohistochemistry, molecular cloning, nonhuman, nucleotide sequence, rabbit, Sarcoptes scabiei, scabies, screening, sequence homology, vaccination, amino acid sequence, animal, chemistry, disease model, gene library, genetics, human, immunology, molecular genetics, parasitology, polymerase chain reaction, Pyroglyphidae, sequence alignment, Acari, Animalia, Arachnida, Astigmata, Canis, Euroglyphus maynei, Hexapoda, Oryctolagus cuniculus, Sarcoptes scabiei type hominis, Allergens, Amino Acid Sequence, Animals, Antigens, Base Sequence, Cloning, Molecular, Disease Models, Animal, DNA, Complementary, Gene Library, Humans, Immune Sera, Immunohistochemistry, Molecular Sequence Data, Polymerase Chain Reaction, Rabbits, RNA, Messenger, Scabies, Sequence Alignment, Sequence Homology, Amino Acid, Vaccines",
author = "P Harumal and Marjorie Morgan and Shelley Walton and Deborah Holt and J Rode and Larry Arlian and Bart Currie and Kemp, {David J}",
year = "2003",
language = "English",
volume = "68",
pages = "54--60",
journal = "The American Journal of Tropical Medicine and Hygiene ",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "1",
}