Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Bart Currie, A Haslem, Talima Pearson, Heidie Hornstra, Benjamin Leadem, Mark Mayo, D Gal, Linda Ward, Daniel Godoy, Brian Spratt, Paul S Keim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.
Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalEmerging Infectious Diseases
Volume15
Issue number2
DOIs
Publication statusPublished - 2009

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Melioidosis
Minisatellite Repeats
Burkholderia pseudomallei
Multilocus Sequence Typing
Disease Outbreaks
Pulsed Field Gel Electrophoresis
Biological Warfare Agents
Drinking Water

Cite this

Currie, Bart ; Haslem, A ; Pearson, Talima ; Hornstra, Heidie ; Leadem, Benjamin ; Mayo, Mark ; Gal, D ; Ward, Linda ; Godoy, Daniel ; Spratt, Brian ; Keim, Paul S. / Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis. In: Emerging Infectious Diseases. 2009 ; Vol. 15, No. 2. pp. 169-174.
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abstract = "Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.",
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Currie, B, Haslem, A, Pearson, T, Hornstra, H, Leadem, B, Mayo, M, Gal, D, Ward, L, Godoy, D, Spratt, B & Keim, PS 2009, 'Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis', Emerging Infectious Diseases, vol. 15, no. 2, pp. 169-174. https://doi.org/10.3201/eid1502.081036

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis. / Currie, Bart; Haslem, A; Pearson, Talima; Hornstra, Heidie; Leadem, Benjamin; Mayo, Mark; Gal, D; Ward, Linda; Godoy, Daniel; Spratt, Brian; Keim, Paul S.

In: Emerging Infectious Diseases, Vol. 15, No. 2, 2009, p. 169-174.

Research output: Contribution to journalArticleResearchpeer-review

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