Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Bart Currie; A Haslem; Talima Pearson; Heidie Hornstra; Benjamin Leadem; Mark Mayo; D Gal; Linda Ward; Daniel Godoy; Brian Spratt; Paul S Keim

doi:10.3201/eid1502.081036

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Bart Currie, A Haslem, Talima Pearson, Heidie Hornstra, Benjamin Leadem, Mark Mayo, D Gal, Linda Ward, Daniel Godoy, Brian Spratt, Paul S Keim

Global and Tropical Health

Research output: Contribution to journal › Article › peer-review

16 Downloads (Pure)

Abstract

Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	Emerging Infectious Diseases
Volume	15
Issue number	2
DOIs	https://doi.org/10.3201/eid1502.081036
Publication status	Published - 2009

Access to Document

10.3201/eid1502.081036Licence: CC BY

5250792-oaFinal published version, 237 KBLicence: CC BY

Cite this

@article{3a53c9346a4f400992ca346de7ba79a9,

title = "Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis",

abstract = "Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.",

keywords = "article, bacterial gene, bacterial strain, bacterium isolate, Burkholderia pseudomallei, epidemic, gene sequence, human, intermethod comparison, melioidosis, multilocus sequence typing, nonhuman, pulsed field gel electrophoresis, strain identification, tandem repeat, Bacterial Typing Techniques, Base Sequence, Disease Outbreaks, Electrophoresis, Gel, Pulsed-Field, Humans, Melioidosis, Minisatellite Repeats, Molecular Sequence Data, Sequence Analysis, DNA",

author = "Bart Currie and A Haslem and Talima Pearson and Heidie Hornstra and Benjamin Leadem and Mark Mayo and D Gal and Linda Ward and Daniel Godoy and Brian Spratt and Keim, {Paul S}",

year = "2009",

doi = "10.3201/eid1502.081036",

language = "English",

volume = "15",

pages = "169--174",

journal = "Emerging Infectious Diseases",

issn = "1080-6040",

publisher = "Centres for Disease Control and Prevention",

number = "2",

}

TY - JOUR

T1 - Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

AU - Currie, Bart

AU - Haslem, A

AU - Pearson, Talima

AU - Hornstra, Heidie

AU - Leadem, Benjamin

AU - Mayo, Mark

AU - Gal, D

AU - Ward, Linda

AU - Godoy, Daniel

AU - Spratt, Brian

AU - Keim, Paul S

PY - 2009

Y1 - 2009

N2 - Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.

AB - Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.

KW - article

KW - bacterial gene

KW - bacterial strain

KW - bacterium isolate

KW - Burkholderia pseudomallei

KW - epidemic

KW - gene sequence

KW - human

KW - intermethod comparison

KW - melioidosis

KW - multilocus sequence typing

KW - nonhuman

KW - pulsed field gel electrophoresis

KW - strain identification

KW - tandem repeat

KW - Bacterial Typing Techniques

KW - Base Sequence

KW - Disease Outbreaks

KW - Electrophoresis, Gel, Pulsed-Field

KW - Humans

KW - Melioidosis

KW - Minisatellite Repeats

KW - Molecular Sequence Data

KW - Sequence Analysis, DNA

UR - http://www.scopus.com/inward/record.url?scp=63849262403&partnerID=8YFLogxK

U2 - 10.3201/eid1502.081036

DO - 10.3201/eid1502.081036

M3 - Article

SN - 1080-6040

VL - 15

SP - 169

EP - 174

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 2

ER -

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this