Increased Allergic Immune Response to Sarcoptes scabiei Antigens in Crusted versus Ordinary Scabies

Shelley Walton, Susan Pizzutto, Amy Slender, Linda Viberg, Deborah Holt, Belinda Hales, David J Kemp, Bart Currie, Jennifer M Rolland, Robyn O'hehir

    Research output: Contribution to journalArticle

    Abstract

    Scabies, a parasitic skin infestation by the burrowing "itch" mite Sarcoptes scabiei, causes significant health problems for children and adults worldwide. Crusted scabies is a particularly severe form of scabies in which mites multiply into the millions, causing extensive skin crusting. The symptoms and signs of scabies suggest host immunity to the scabies mite, but the specific resistant response in humans remains largely uncharacterized. We used 4 scabies mite recombinant proteins with sequence homology to extensively studied house dust mite allergens to investigate a differential immune response between ordinary scabies and the debilitating crusted form of the disease. Subjects with either disease form showed serum IgE against recombinant S. scabiei cysteine and serine proteases and apolipoprotein, whereas naive subjects showed minimal IgE reactivity. Significantly (P < 0.05) greater serum IgE and IgG4 binding to mite apolipoprotein occurred in subjects with crusted scabies than in those with ordinary scabies. Both subject groups showed strong proliferative responses (peripheral blood mononuclear cells) to the scabies antigens, but the crusted scabies group showed increased secretion of the Th2 cytokines interleukin 5 (IL-5) and IL-13 and decreased Th1 cytokine gamma interferon (IFN-?) in response to the active cysteine protease. These data confirm that a nonprotective allergic response occurs in the crusted disease form and demonstrate that clinical severity is associated with differences in the type and magnitude of the antibody and cellular responses to scabies proteins. A quantitative IgE inhibition assay identified IgE immunoreactivity of scabies mite antigens distinct from that of house dust mite antigens, which is potentially important for specific scabies diagnosis and therapy. Copyright � 2010, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)1428-4238
    Number of pages2811
    JournalClinical and Vaccine Immunology
    Volume17
    Issue number9
    DOIs
    Publication statusPublished - 2010

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    Walton, S., Pizzutto, S., Slender, A., Viberg, L., Holt, D., Hales, B., Kemp, D. J., Currie, B., Rolland, J. M., & O'hehir, R. (2010). Increased Allergic Immune Response to Sarcoptes scabiei Antigens in Crusted versus Ordinary Scabies. Clinical and Vaccine Immunology, 17(9), 1428-4238. https://doi.org/10.1128/CVI.00195-10