Induction and Kinetics of Complement-Fixing Antibodies Against Plasmodium vivax Merozoite Surface Protein 3α and Relationship With Immunoglobulin G Subclasses and Immunoglobulin M

Damian A. Oyong, Danny W. Wilson, Bridget E. Barber, Timothy William, Jianlin Jiang, Mary R. Galinski, Freya J.I. Fowkes, Matthew J. Grigg, James G. Beeson, Nicholas M. Anstey, Michelle J. Boyle

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Background: Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterized for Plasmodium vivax malaria. P. vivax merozoite surface protein 3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate.

    Methods: Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days after drug treatment. Complement-fixing antibodies and immunoglobulin M and G (IgM and IgG), targeting 3 distinctive regions of PvMSP3α, were measured by means of enzyme-linked immunosorbent assay.

    Results: The seroprevalence of complement-fixing antibodies was highest against the PvMSP3α central region (77.6%). IgG1, IgG3, and IgM were significantly correlated with C1q fixation, and both purified IgG and IgM were capable of mediating C1q fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Levels of functional antibodies increased after acute infection through 7 days after treatment but rapidly waned by day 28.

    Conclusion: Our study demonstrates that PvMSP3α antibodies acquired during P. vivax infection can mediate complement fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria.

    Original languageEnglish
    Pages (from-to)1950-1961
    Number of pages12
    JournalThe Journal of infectious diseases
    Volume220
    Issue number12
    Early online date17 Aug 2019
    DOIs
    Publication statusPublished - 15 Dec 2019

    Fingerprint

    Plasmodium vivax
    Immunoglobulin M
    Immunoglobulin G
    Antibodies
    Vivax Malaria
    Malaysia
    Plasmodium merozoite surface protein 3
    Falciparum Malaria
    Seroepidemiologic Studies
    Adaptive Immunity
    Infection
    Malaria
    Antibody Formation
    Immunity
    Vaccines
    Age Groups
    Enzyme-Linked Immunosorbent Assay
    Therapeutics

    Cite this

    Oyong, Damian A. ; Wilson, Danny W. ; Barber, Bridget E. ; William, Timothy ; Jiang, Jianlin ; Galinski, Mary R. ; Fowkes, Freya J.I. ; Grigg, Matthew J. ; Beeson, James G. ; Anstey, Nicholas M. ; Boyle, Michelle J. / Induction and Kinetics of Complement-Fixing Antibodies Against Plasmodium vivax Merozoite Surface Protein 3α and Relationship With Immunoglobulin G Subclasses and Immunoglobulin M. In: The Journal of infectious diseases. 2019 ; Vol. 220, No. 12. pp. 1950-1961.
    @article{59b2f5b2b0fa494da6812767603886cb,
    title = "Induction and Kinetics of Complement-Fixing Antibodies Against Plasmodium vivax Merozoite Surface Protein 3α and Relationship With Immunoglobulin G Subclasses and Immunoglobulin M",
    abstract = "Background: Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterized for Plasmodium vivax malaria. P. vivax merozoite surface protein 3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate. Methods: Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days after drug treatment. Complement-fixing antibodies and immunoglobulin M and G (IgM and IgG), targeting 3 distinctive regions of PvMSP3α, were measured by means of enzyme-linked immunosorbent assay. Results: The seroprevalence of complement-fixing antibodies was highest against the PvMSP3α central region (77.6{\%}). IgG1, IgG3, and IgM were significantly correlated with C1q fixation, and both purified IgG and IgM were capable of mediating C1q fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Levels of functional antibodies increased after acute infection through 7 days after treatment but rapidly waned by day 28. Conclusion: Our study demonstrates that PvMSP3α antibodies acquired during P. vivax infection can mediate complement fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria.",
    keywords = "Plasmodium vivax, Complement-fixing antibodies, malaria, PvMSP3α",
    author = "Oyong, {Damian A.} and Wilson, {Danny W.} and Barber, {Bridget E.} and Timothy William and Jianlin Jiang and Galinski, {Mary R.} and Fowkes, {Freya J.I.} and Grigg, {Matthew J.} and Beeson, {James G.} and Anstey, {Nicholas M.} and Boyle, {Michelle J.}",
    year = "2019",
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    Induction and Kinetics of Complement-Fixing Antibodies Against Plasmodium vivax Merozoite Surface Protein 3α and Relationship With Immunoglobulin G Subclasses and Immunoglobulin M. / Oyong, Damian A.; Wilson, Danny W.; Barber, Bridget E.; William, Timothy; Jiang, Jianlin; Galinski, Mary R.; Fowkes, Freya J.I.; Grigg, Matthew J.; Beeson, James G.; Anstey, Nicholas M.; Boyle, Michelle J.

    In: The Journal of infectious diseases, Vol. 220, No. 12, 15.12.2019, p. 1950-1961.

    Research output: Contribution to journalArticleResearchpeer-review

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    T1 - Induction and Kinetics of Complement-Fixing Antibodies Against Plasmodium vivax Merozoite Surface Protein 3α and Relationship With Immunoglobulin G Subclasses and Immunoglobulin M

    AU - Oyong, Damian A.

    AU - Wilson, Danny W.

    AU - Barber, Bridget E.

    AU - William, Timothy

    AU - Jiang, Jianlin

    AU - Galinski, Mary R.

    AU - Fowkes, Freya J.I.

    AU - Grigg, Matthew J.

    AU - Beeson, James G.

    AU - Anstey, Nicholas M.

    AU - Boyle, Michelle J.

    PY - 2019/12/15

    Y1 - 2019/12/15

    N2 - Background: Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterized for Plasmodium vivax malaria. P. vivax merozoite surface protein 3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate. Methods: Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days after drug treatment. Complement-fixing antibodies and immunoglobulin M and G (IgM and IgG), targeting 3 distinctive regions of PvMSP3α, were measured by means of enzyme-linked immunosorbent assay. Results: The seroprevalence of complement-fixing antibodies was highest against the PvMSP3α central region (77.6%). IgG1, IgG3, and IgM were significantly correlated with C1q fixation, and both purified IgG and IgM were capable of mediating C1q fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Levels of functional antibodies increased after acute infection through 7 days after treatment but rapidly waned by day 28. Conclusion: Our study demonstrates that PvMSP3α antibodies acquired during P. vivax infection can mediate complement fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria.

    AB - Background: Complement-fixing antibodies are important mediators of protection against Plasmodium falciparum malaria. However, complement-fixing antibodies remain uncharacterized for Plasmodium vivax malaria. P. vivax merozoite surface protein 3α (PvMSP3α) is a target of acquired immunity and a potential vaccine candidate. Methods: Plasma from children and adults with P. vivax malaria in Sabah, Malaysia, were collected during acute infection, 7 and 28 days after drug treatment. Complement-fixing antibodies and immunoglobulin M and G (IgM and IgG), targeting 3 distinctive regions of PvMSP3α, were measured by means of enzyme-linked immunosorbent assay. Results: The seroprevalence of complement-fixing antibodies was highest against the PvMSP3α central region (77.6%). IgG1, IgG3, and IgM were significantly correlated with C1q fixation, and both purified IgG and IgM were capable of mediating C1q fixation to PvMSP3α. Complement-fixing antibody levels were similar between age groups, but IgM was predominant in children and IgG3 more prevalent in adults. Levels of functional antibodies increased after acute infection through 7 days after treatment but rapidly waned by day 28. Conclusion: Our study demonstrates that PvMSP3α antibodies acquired during P. vivax infection can mediate complement fixation and shows the important influence of age in shaping these specific antibody responses. Further studies are warranted to understand the role of these functional antibodies in protective immunity against P. vivax malaria.

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