In this study, linkages were examined between movement and spawning behaviour for golden perch Macquaria ambigua in a lowland river by integrating acoustic telemetry and egg and larval drift sampling over 4 years. Movement was strongly seasonal, being most prevalent during the spawning season (spring to early summer), and occurred primarily downstream into the lower river reaches during elevated flows. A very strong association was found between the occurrence of spawning and long-distance M. ambigua movement. The results also revealed that targeted environmental water allocation can promote movement and spawning of this species. By integrating multiple analytical approaches and focusing on key life-history events, this study provides an improved picture of the life history and flow requirements of M. ambigua. The findings can help guide the development of effective environmental flow recommendations.