Investigation of tunichrome and its relation to vanadium in living ascidian blood cells and their lysates

S. W. Taylor; David Parry; C. J. Hawkins; J. H. Swinehart

doi:10.1016/0300-9629(93)90249-4

Investigation of tunichrome and its relation to vanadium in living ascidian blood cells and their lysates

S. W. Taylor, David Parry, C. J. Hawkins, J. H. Swinehart

Research output: Contribution to journal › Comment/debate

6 Citations (Scopus)

Abstract

1. Results presented in this paper show that there is no evidence for tunichrome in intact cells of the two Ascidiidae species Ascidia ceralodes or Phallusia julinea.

2. The yellow fluorescence of ascidian blood cells cannot be unequivocally ascribed to the yellow fluorescent tunichrome and should not be used as evidence for the compound in vivo.

3. Spectrofluorometric emission peaks reported by previous workers as evidence of tunichrome in vivo were in the same positions as water Raman bands.

4. Yellow fluorescence has been observed in the blood cells of species in which tunichrome has not been found, for example Ciona intestinalis and Pyura stolonifera.

5. The accumulated data suggest that both the lysis of morula [low in vanadium (V) and high in tunichrome precursor contents] and compartment-signet ring (high in V content) cells are necessary for the formation of tuniehrome.

Original language	English
Pages (from-to)	531-536
Number of pages	6
Journal	Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology
Volume	106
Issue number	3
DOIs	https://doi.org/10.1016/0300-9629(93)90249-4
Publication status	Published - 1993

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title = "Investigation of tunichrome and its relation to vanadium in living ascidian blood cells and their lysates",

abstract = "1. Results presented in this paper show that there is no evidence for tunichrome in intact cells of the two Ascidiidae species Ascidia ceralodes or Phallusia julinea.2. The yellow fluorescence of ascidian blood cells cannot be unequivocally ascribed to the yellow fluorescent tunichrome and should not be used as evidence for the compound in vivo.3. Spectrofluorometric emission peaks reported by previous workers as evidence of tunichrome in vivo were in the same positions as water Raman bands.4. Yellow fluorescence has been observed in the blood cells of species in which tunichrome has not been found, for example Ciona intestinalis and Pyura stolonifera.5. The accumulated data suggest that both the lysis of morula [low in vanadium (V) and high in tunichrome precursor contents] and compartment-signet ring (high in V content) cells are necessary for the formation of tuniehrome.",

author = "Taylor, {S. W.} and David Parry and Hawkins, {C. J.} and Swinehart, {J. H.}",

year = "1993",

doi = "10.1016/0300-9629(93)90249-4",

language = "English",

volume = "106",

pages = "531--536",

journal = "Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology",

issn = "0300-9629",

publisher = "Elsevier Science",

number = "3",

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TY - JOUR

T1 - Investigation of tunichrome and its relation to vanadium in living ascidian blood cells and their lysates

AU - Taylor, S. W.

AU - Parry, David

AU - Hawkins, C. J.

AU - Swinehart, J. H.

PY - 1993

Y1 - 1993

N2 - 1. Results presented in this paper show that there is no evidence for tunichrome in intact cells of the two Ascidiidae species Ascidia ceralodes or Phallusia julinea.2. The yellow fluorescence of ascidian blood cells cannot be unequivocally ascribed to the yellow fluorescent tunichrome and should not be used as evidence for the compound in vivo.3. Spectrofluorometric emission peaks reported by previous workers as evidence of tunichrome in vivo were in the same positions as water Raman bands.4. Yellow fluorescence has been observed in the blood cells of species in which tunichrome has not been found, for example Ciona intestinalis and Pyura stolonifera.5. The accumulated data suggest that both the lysis of morula [low in vanadium (V) and high in tunichrome precursor contents] and compartment-signet ring (high in V content) cells are necessary for the formation of tuniehrome.

AB - 1. Results presented in this paper show that there is no evidence for tunichrome in intact cells of the two Ascidiidae species Ascidia ceralodes or Phallusia julinea.2. The yellow fluorescence of ascidian blood cells cannot be unequivocally ascribed to the yellow fluorescent tunichrome and should not be used as evidence for the compound in vivo.3. Spectrofluorometric emission peaks reported by previous workers as evidence of tunichrome in vivo were in the same positions as water Raman bands.4. Yellow fluorescence has been observed in the blood cells of species in which tunichrome has not been found, for example Ciona intestinalis and Pyura stolonifera.5. The accumulated data suggest that both the lysis of morula [low in vanadium (V) and high in tunichrome precursor contents] and compartment-signet ring (high in V content) cells are necessary for the formation of tuniehrome.

U2 - 10.1016/0300-9629(93)90249-4

DO - 10.1016/0300-9629(93)90249-4

M3 - Comment/debate

SN - 0300-9629

VL - 106

SP - 531

EP - 536

JO - Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology

IS - 3

ER -

Investigation of tunichrome and its relation to vanadium in living ascidian blood cells and their lysates

Abstract

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