Plasmodium vivax malaria serological exposure markers: Assessing the degree and implications of cross-reactivity with P. knowlesi

Rhea J. Longley, Matthew J. Grigg, Kael Schoffer, Thomas Obadia, Stephanie Hyslop, Kim A. Piera, Narimane Nekkab, Ramin Mazhari, Eizo Takashima, Takafumi Tsuboi, Matthias Harbers, Kevin Tetteh, Chris Drakeley, Chetan E. Chitnis, Julie Healer, Wai Hong Tham, Jetsumon Sattabongkot, Michael T. White, Daniel J. Cooper, Giri S. RajahramBridget E. Barber, Timothy William, Nicholas M. Anstey, Ivo Mueller

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Abstract

Serological markers are a promising tool for surveillance and targeted interventions for Plasmodium vivax malaria. P. vivax is closely related to the zoonotic parasite P. knowlesi, which also infects humans. P. vivax and P. knowlesi are co-endemic across much of South East Asia, making it important to design serological markers that minimize cross-reactivity in this region. To determine the degree of IgG cross-reactivity against a panel of P. vivax serological markers, we assayed samples from human patients with P. knowlesi malaria. IgG antibody reactivity is high against P. vivax proteins with high sequence identity with their P. knowlesi ortholog. IgG reactivity peaks at 7 days post-P. knowlesi infection and is short-lived, with minimal responses 1 year post-infection. We designed a panel of eight P. vivax proteins with low levels of cross-reactivity with P. knowlesi. This panel can accurately classify recent P. vivax infections while reducing misclassification of recent P. knowlesi infections.

Original languageEnglish
Article number100662
Pages (from-to)1-17
Number of pages17
JournalCell reports. Medicine
Volume3
Issue number6
DOIs
Publication statusPublished - 21 Jun 2022

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