Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation

Md Robi Islam, Angelo Keramidas, Li Xu, Nela Durisic, Pankaj Sah, Joseph W Lynch

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The ability to control neuronal activation is rapidly advancing our understanding of brain function and is widely viewed as having eventual therapeutic application. Although several highly effective optogenetic, optochemical genetic, and chemogenetic techniques have been developed for this purpose, new approaches may provide better solutions for addressing particular questions and would increase the number of neuronal populations that can be controlled independently. An early chemogenetic neuronal silencing method employed a glutamate receptor Cl−channel engineered for activation by 1−3 nM ivermectin. This construct has been validated in vivo. Here, we sought to develop cation-permeable ivermectin-gated receptors that were either maximally Ca2+ -permeable so as to induce neuro-excitotoxic cell death or minimally Ca2+ -permeable so as to depolarize neurons with minimal excitotoxic risk. Our constructs were based on the human α 1 glycine receptor Cl− channel due to its high conductance, human origin, high ivermectin sensitivity (once mutated), and because pore mutations that render it permeable to Na+ alone or Na+ plus Ca2+ are well characterized. We developed a Ca2+ -impermeable excitatory receptor by introducing the F207A/P-2 ′ Δ/A-1 ′ E/T13 ′ V/A288G mutations and a Ca2+ -permeable excitatory receptor by introducing the F207A/A-1 ′ E/A288G mutations. The latter receptor efficiently induces cell death and strongly depolarizes neurons at nanomolar ivermectin concentrations.
Original languageEnglish
Pages (from-to)1647-1657
Number of pages11
JournalACS Chemical Neuroscience
Volume7
Issue number12
DOIs
Publication statusPublished - 2016
Externally publishedYes

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Glycine Receptors
Ivermectin
Cations
Cell death
Mutation
Neurons
Cell Death
Chemical activation
Optogenetics
Genetic Techniques
Glutamate Receptors
Brain
Population

Cite this

Islam, Md Robi ; Keramidas, Angelo ; Xu, Li ; Durisic, Nela ; Sah, Pankaj ; Lynch, Joseph W. / Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation. In: ACS Chemical Neuroscience. 2016 ; Vol. 7, No. 12. pp. 1647-1657.
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abstract = "The ability to control neuronal activation is rapidly advancing our understanding of brain function and is widely viewed as having eventual therapeutic application. Although several highly effective optogenetic, optochemical genetic, and chemogenetic techniques have been developed for this purpose, new approaches may provide better solutions for addressing particular questions and would increase the number of neuronal populations that can be controlled independently. An early chemogenetic neuronal silencing method employed a glutamate receptor Cl−channel engineered for activation by 1−3 nM ivermectin. This construct has been validated in vivo. Here, we sought to develop cation-permeable ivermectin-gated receptors that were either maximally Ca2+ -permeable so as to induce neuro-excitotoxic cell death or minimally Ca2+ -permeable so as to depolarize neurons with minimal excitotoxic risk. Our constructs were based on the human α 1 glycine receptor Cl− channel due to its high conductance, human origin, high ivermectin sensitivity (once mutated), and because pore mutations that render it permeable to Na+ alone or Na+ plus Ca2+ are well characterized. We developed a Ca2+ -impermeable excitatory receptor by introducing the F207A/P-2 ′ Δ/A-1 ′ E/T13 ′ V/A288G mutations and a Ca2+ -permeable excitatory receptor by introducing the F207A/A-1 ′ E/A288G mutations. The latter receptor efficiently induces cell death and strongly depolarizes neurons at nanomolar ivermectin concentrations.",
author = "Islam, {Md Robi} and Angelo Keramidas and Li Xu and Nela Durisic and Pankaj Sah and Lynch, {Joseph W}",
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Islam, MR, Keramidas, A, Xu, L, Durisic, N, Sah, P & Lynch, JW 2016, 'Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation', ACS Chemical Neuroscience, vol. 7, no. 12, pp. 1647-1657. https://doi.org/10.1021/acschemneuro.6b00168

Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation. / Islam, Md Robi; Keramidas, Angelo ; Xu, Li; Durisic, Nela; Sah, Pankaj; Lynch, Joseph W.

In: ACS Chemical Neuroscience, Vol. 7, No. 12, 2016, p. 1647-1657.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Islam, Md Robi

AU - Keramidas, Angelo

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AU - Durisic, Nela

AU - Sah, Pankaj

AU - Lynch, Joseph W

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AB - The ability to control neuronal activation is rapidly advancing our understanding of brain function and is widely viewed as having eventual therapeutic application. Although several highly effective optogenetic, optochemical genetic, and chemogenetic techniques have been developed for this purpose, new approaches may provide better solutions for addressing particular questions and would increase the number of neuronal populations that can be controlled independently. An early chemogenetic neuronal silencing method employed a glutamate receptor Cl−channel engineered for activation by 1−3 nM ivermectin. This construct has been validated in vivo. Here, we sought to develop cation-permeable ivermectin-gated receptors that were either maximally Ca2+ -permeable so as to induce neuro-excitotoxic cell death or minimally Ca2+ -permeable so as to depolarize neurons with minimal excitotoxic risk. Our constructs were based on the human α 1 glycine receptor Cl− channel due to its high conductance, human origin, high ivermectin sensitivity (once mutated), and because pore mutations that render it permeable to Na+ alone or Na+ plus Ca2+ are well characterized. We developed a Ca2+ -impermeable excitatory receptor by introducing the F207A/P-2 ′ Δ/A-1 ′ E/T13 ′ V/A288G mutations and a Ca2+ -permeable excitatory receptor by introducing the F207A/A-1 ′ E/A288G mutations. The latter receptor efficiently induces cell death and strongly depolarizes neurons at nanomolar ivermectin concentrations.

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JO - ACS Chemical Neuroscience

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Islam MR, Keramidas A, Xu L, Durisic N, Sah P, Lynch JW. Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation. ACS Chemical Neuroscience. 2016;7(12):1647-1657. https://doi.org/10.1021/acschemneuro.6b00168

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