Kanniadu goats of Tamilnadu, India: genetic characterisation through microsatellite markers

K Thilagam, Rama Jayaraj, S N Sivaselvam, S M Karthickeyan, P Thangaraju

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Abstract

Characterisation of Kanniadu goats was done using microsatellite markers. The genomic DNA from 50 unrelated Kanniadu goats were PCR-amplified with a panel of 20 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining.
The number of alleles ranged from 5 to 14 with allele sizes ranging from 90 to 222bp. The allele frequencies ranged from 0.0106 to 0.4480. Polymorphism information content ranged from 0.5710 to 0.8570. Except four loci, thepopulation was not in Hardy-Weinberg equilibrium. The observed heterozygosity ranged from 0.7142 to 0.9778 while the expected heterozygosity ranged from 0. 6390 to 0.8702, indicating the heterogenous nature of the population distributed in the breeding tract.
Original languageEnglish
Number of pages5
JournalLivestock Research for Rural Development
Volume18
Issue number10
Publication statusPublished - 2006
Externally publishedYes

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    Thilagam, K., Jayaraj, R., Sivaselvam, S. N., Karthickeyan, S. M., & Thangaraju, P. (2006). Kanniadu goats of Tamilnadu, India: genetic characterisation through microsatellite markers. Livestock Research for Rural Development, 18(10).