Kanniadu goats of Tamilnadu, India

genetic characterisation through microsatellite markers

K Thilagam, Rama Jayaraj, S N Sivaselvam, S M Karthickeyan, P Thangaraju

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Characterisation of Kanniadu goats was done using microsatellite markers. The genomic DNA from 50 unrelated Kanniadu goats were PCR-amplified with a panel of 20 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining.
The number of alleles ranged from 5 to 14 with allele sizes ranging from 90 to 222bp. The allele frequencies ranged from 0.0106 to 0.4480. Polymorphism information content ranged from 0.5710 to 0.8570. Except four loci, thepopulation was not in Hardy-Weinberg equilibrium. The observed heterozygosity ranged from 0.7142 to 0.9778 while the expected heterozygosity ranged from 0. 6390 to 0.8702, indicating the heterogenous nature of the population distributed in the breeding tract.
Original languageEnglish
Number of pages5
JournalLivestock Research for Rural Development
Volume18
Issue number10
Publication statusPublished - 2006
Externally publishedYes

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heterozygosity
goats
microsatellite repeats
alleles
India
gene frequency
polyacrylamide gel electrophoresis
genetic polymorphism
genomics
loci
breeding
DNA
silver staining

Cite this

Thilagam, K ; Jayaraj, Rama ; Sivaselvam, S N ; Karthickeyan, S M ; Thangaraju, P. / Kanniadu goats of Tamilnadu, India : genetic characterisation through microsatellite markers. In: Livestock Research for Rural Development. 2006 ; Vol. 18, No. 10.
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abstract = "Characterisation of Kanniadu goats was done using microsatellite markers. The genomic DNA from 50 unrelated Kanniadu goats were PCR-amplified with a panel of 20 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining.The number of alleles ranged from 5 to 14 with allele sizes ranging from 90 to 222bp. The allele frequencies ranged from 0.0106 to 0.4480. Polymorphism information content ranged from 0.5710 to 0.8570. Except four loci, thepopulation was not in Hardy-Weinberg equilibrium. The observed heterozygosity ranged from 0.7142 to 0.9778 while the expected heterozygosity ranged from 0. 6390 to 0.8702, indicating the heterogenous nature of the population distributed in the breeding tract.",
author = "K Thilagam and Rama Jayaraj and Sivaselvam, {S N} and Karthickeyan, {S M} and P Thangaraju",
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Kanniadu goats of Tamilnadu, India : genetic characterisation through microsatellite markers. / Thilagam, K; Jayaraj, Rama; Sivaselvam, S N; Karthickeyan, S M; Thangaraju, P.

In: Livestock Research for Rural Development, Vol. 18, No. 10, 2006.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Kanniadu goats of Tamilnadu, India

T2 - genetic characterisation through microsatellite markers

AU - Thilagam, K

AU - Jayaraj, Rama

AU - Sivaselvam, S N

AU - Karthickeyan, S M

AU - Thangaraju, P

PY - 2006

Y1 - 2006

N2 - Characterisation of Kanniadu goats was done using microsatellite markers. The genomic DNA from 50 unrelated Kanniadu goats were PCR-amplified with a panel of 20 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining.The number of alleles ranged from 5 to 14 with allele sizes ranging from 90 to 222bp. The allele frequencies ranged from 0.0106 to 0.4480. Polymorphism information content ranged from 0.5710 to 0.8570. Except four loci, thepopulation was not in Hardy-Weinberg equilibrium. The observed heterozygosity ranged from 0.7142 to 0.9778 while the expected heterozygosity ranged from 0. 6390 to 0.8702, indicating the heterogenous nature of the population distributed in the breeding tract.

AB - Characterisation of Kanniadu goats was done using microsatellite markers. The genomic DNA from 50 unrelated Kanniadu goats were PCR-amplified with a panel of 20 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining.The number of alleles ranged from 5 to 14 with allele sizes ranging from 90 to 222bp. The allele frequencies ranged from 0.0106 to 0.4480. Polymorphism information content ranged from 0.5710 to 0.8570. Except four loci, thepopulation was not in Hardy-Weinberg equilibrium. The observed heterozygosity ranged from 0.7142 to 0.9778 while the expected heterozygosity ranged from 0. 6390 to 0.8702, indicating the heterogenous nature of the population distributed in the breeding tract.

M3 - Article

VL - 18

JO - Livestock Research for Rural Development

JF - Livestock Research for Rural Development

SN - 0121-3784

IS - 10

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