Long PCR-ribotyping of nontypeable Haemophilus influenzae

H. C. Smith-Vaughan; K. S. Sriprakash; J. D. Mathews; D. J. Kemp

doi:10.1128/jcm.33.5.1192-1195.1995

Long PCR-ribotyping of nontypeable Haemophilus influenzae

H. C. Smith-Vaughan, K. S. Sriprakash, J. D. Mathews, D. J. Kemp

Research output: Contribution to journal › Article › peer-review

Abstract

PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.

Original language	English
Pages (from-to)	1192-1195
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	33
Issue number	5
DOIs	https://doi.org/10.1128/jcm.33.5.1192-1195.1995
Publication status	Published - 1995

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10.1128/jcm.33.5.1192-1195.1995

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abstract = "PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.",

author = "Smith-Vaughan, {H. C.} and Sriprakash, {K. S.} and Mathews, {J. D.} and Kemp, {D. J.}",

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AU - Mathews, J. D.

AU - Kemp, D. J.

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Long PCR-ribotyping of nontypeable Haemophilus influenzae

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