Loss of complement regulatory proteins on red blood cells in mild malarial anaemia and in Plasmodium falciparum induced blood-stage infection

Damian A. Oyong, Jessica R. Loughland, Arya Sheelanair, Dean Andrew, Fabian D.L. Rivera, Kim A. Piera, Timothy William, Matthew J. Grigg, Bridget E. Barber, Ashraful Haque, Christian R. Engwerda, James S. McCarthy, Nicholas M. Anstey, Michelle J. Boyle

    Research output: Contribution to journalArticle

    20 Downloads (Pure)

    Abstract

    Background: Anaemia is a major consequence of malaria, caused by the removal of both infected and uninfected red blood cells (RBCs) from the circulation. Complement activation and reduced expression of complement regulatory proteins (CRPs) on RBCs are an important pathogenic mechanism in severe malarial anaemia in both Plasmodium falciparum and Plasmodium vivax infection. However, little is known about loss of CRPs on RBCs during mild malarial anaemia and in low-density infection. 

    Methods: The expression of CRP CR1, CD55, CD59, and the phagocytic regulator CD47, on uninfected normocytes and reticulocytes were assessed in individuals from two study populations: (1) P. falciparum and P. vivax-infected patients from a low transmission setting in Sabah, Malaysia; and, (2) malaria-naïve volunteers undergoing P. falciparum induced blood-stage malaria (IBSM). For clinical infections, individuals were categorized into anaemia severity categories based on haemoglobin levels. For IBSM, associations between CRPs and haemoglobin level were investigated. 

    Results: CRP expression on RBC was lower in Malaysian individuals with P. falciparum and P. vivax mild malarial anaemia compared to healthy controls. CRP expression was also reduced on RBCs from volunteers during IBSM. Reduction occurred on normocytes and reticulocytes. However, there was no significant association between reduced CRPs and haemoglobin during IBSM. 

    Conclusions: Removal of CRPs occurs on both RBCs and reticulocytes during Plasmodium infection even in mild malarial anaemia and at low levels of parasitaemia.

    Original languageEnglish
    Article number312
    Pages (from-to)1-11
    Number of pages11
    JournalMalaria Journal
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 18 Sep 2019

    Fingerprint Dive into the research topics of 'Loss of complement regulatory proteins on red blood cells in mild malarial anaemia and in Plasmodium falciparum induced blood-stage infection'. Together they form a unique fingerprint.

  • Cite this

    Oyong, D. A., Loughland, J. R., Sheelanair, A., Andrew, D., Rivera, F. D. L., Piera, K. A., William, T., Grigg, M. J., Barber, B. E., Haque, A., Engwerda, C. R., McCarthy, J. S., Anstey, N. M., & Boyle, M. J. (2019). Loss of complement regulatory proteins on red blood cells in mild malarial anaemia and in Plasmodium falciparum induced blood-stage infection. Malaria Journal, 18(1), 1-11. [312]. https://doi.org/10.1186/s12936-019-2962-0