Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia

K Chambers, Anna Padovan, Belinda Alvarez de Glasby, Karen Gibb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.
    Original languageEnglish
    Pages (from-to)716-725
    Number of pages10
    JournalMarine and Freshwater Research
    Volume64
    Issue number8
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Porifera
    morphs
    sponge
    Moon
    harbor
    bacterial communities
    gene
    targeting
    cytochrome
    electrokinesis
    Denaturing Gradient Gel Electrophoresis
    gel
    Electron Transport Complex IV
    denaturing gradient gel electrophoresis
    rRNA Genes
    cytochrome-c oxidase
    genes
    ribosomal RNA
    taxonomy
    Genes

    Cite this

    @article{425014f9e848460ab37963d8667e72d0,
    title = "Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia",
    abstract = "The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.",
    keywords = "data acquisition, gene expression, microbial community, species diversity, sponge, taxonomy, Australia, Darwin Harbour, Northern Territory",
    author = "K Chambers and Anna Padovan and {Alvarez de Glasby}, Belinda and Karen Gibb",
    year = "2013",
    doi = "10.1071/MF12226",
    language = "English",
    volume = "64",
    pages = "716--725",
    journal = "Marine & Freshwater Research",
    issn = "0067-1940",
    publisher = "CSIRO Publishing",
    number = "8",

    }

    Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia. / Chambers, K; Padovan, Anna; Alvarez de Glasby, Belinda; Gibb, Karen.

    In: Marine and Freshwater Research, Vol. 64, No. 8, 2013, p. 716-725.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia

    AU - Chambers, K

    AU - Padovan, Anna

    AU - Alvarez de Glasby, Belinda

    AU - Gibb, Karen

    PY - 2013

    Y1 - 2013

    N2 - The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.

    AB - The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.

    KW - data acquisition

    KW - gene expression

    KW - microbial community

    KW - species diversity

    KW - sponge

    KW - taxonomy

    KW - Australia

    KW - Darwin Harbour

    KW - Northern Territory

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    DO - 10.1071/MF12226

    M3 - Article

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    EP - 725

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    SN - 0067-1940

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