Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia

K Chambers; Anna Padovan; Belinda Alvarez de Glasby; Karen Gibb

doi:10.1071/MF12226

Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia

K Chambers, Anna Padovan, Belinda Alvarez de Glasby, Karen Gibb

Research output: Contribution to journal › Article

Abstract

The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.

Original language	English
Pages (from-to)	716-725
Number of pages	10
Journal	Marine and Freshwater Research
Volume	64
Issue number	8
DOIs	https://doi.org/10.1071/MF12226
Publication status	Published - 2013

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Chambers, K., Padovan, A., Alvarez de Glasby, B., & Gibb, K. (2013). Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia. Marine and Freshwater Research, 64(8), 716-725. https://doi.org/10.1071/MF12226

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title = "Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia",

abstract = "The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla. ",

keywords = "data acquisition, gene expression, microbial community, species diversity, sponge, taxonomy, Australia, Darwin Harbour, Northern Territory",

author = "K Chambers and Anna Padovan and {Alvarez de Glasby}, Belinda and Karen Gibb",

year = "2013",

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volume = "64",

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journal = "Marine & Freshwater Research",

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N2 - The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.

AB - The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.

KW - data acquisition

KW - gene expression

KW - microbial community

KW - species diversity

KW - sponge

KW - taxonomy

KW - Australia

KW - Darwin Harbour

KW - Northern Territory

U2 - 10.1071/MF12226

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JO - Marine & Freshwater Research

JF - Marine & Freshwater Research

SN - 0067-1940

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