Microbial sulfate reduction under sequentially acidic conditions in an upflow anaerobic packed bed bioreactor

T Jong, David Parry

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The aim of this study was to operate an upflow anaerobic packed bed reactor (UAPB) containing sulfate reducing bacteria (SRB) under acidic conditions similar to those found in acid mine drainage (AMD). The UAPB was filled with sand and operated under continuous flow at progressively lower pH and was shown to be capable of supporting sulfate reduction at pH values of 6.0, 5.0, 4.5, 4.0 and 3.5 in a synthetic medium containing 53.5 mmol l-1 lactate. Sulfate reduction rates of 553-1052 mmol m-3 d-1 were obtained when the influent solution pH was progressively lowered from pH 6.0 to 4.0, under an optimal flow rate of 2.61 ml min-1. When the influent pH was further lowered to pH 3.5, sulfate reduction was substantially reduced with only about 1% sulfate removed at a rate of 3.35 mmol m-3 d-1 after 20 days of operation. However, viable SRB were recovered from the column, indicating that the SRB population was capable of surviving and metabolizing at low levels even at pH 3.5 conditions for at least 20 days. The changes in conductivity in the SRB column did not always occur with changes in pH and redox potential, suggesting that conductivity measurements may be more sensitive to SRB activity and could be used as an additional tool for monitoring SRB activity. The bioreactor containing SRB was able to reduce sulfate and generate alkalinity even when challenged with influent as low as pH 3.5, indicating that such treatment systems have potential for bioremediating highly acidic, sulfate contaminated waste waters. � 2006 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)2561-2571
    Number of pages11
    JournalWater Research
    Volume40
    Issue number13
    Publication statusPublished - 2006

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    sulfate-reducing bacterium
    Packed beds
    Bioreactors
    bioreactor
    sulfate
    Bacteria
    conductivity
    acid mine drainage
    redox potential
    Sulfates
    alkalinity
    sand
    Alkalinity
    monitoring
    Drainage
    rate
    Wastewater
    Sand
    Flow rate

    Cite this

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    abstract = "The aim of this study was to operate an upflow anaerobic packed bed reactor (UAPB) containing sulfate reducing bacteria (SRB) under acidic conditions similar to those found in acid mine drainage (AMD). The UAPB was filled with sand and operated under continuous flow at progressively lower pH and was shown to be capable of supporting sulfate reduction at pH values of 6.0, 5.0, 4.5, 4.0 and 3.5 in a synthetic medium containing 53.5 mmol l-1 lactate. Sulfate reduction rates of 553-1052 mmol m-3 d-1 were obtained when the influent solution pH was progressively lowered from pH 6.0 to 4.0, under an optimal flow rate of 2.61 ml min-1. When the influent pH was further lowered to pH 3.5, sulfate reduction was substantially reduced with only about 1{\%} sulfate removed at a rate of 3.35 mmol m-3 d-1 after 20 days of operation. However, viable SRB were recovered from the column, indicating that the SRB population was capable of surviving and metabolizing at low levels even at pH 3.5 conditions for at least 20 days. The changes in conductivity in the SRB column did not always occur with changes in pH and redox potential, suggesting that conductivity measurements may be more sensitive to SRB activity and could be used as an additional tool for monitoring SRB activity. The bioreactor containing SRB was able to reduce sulfate and generate alkalinity even when challenged with influent as low as pH 3.5, indicating that such treatment systems have potential for bioremediating highly acidic, sulfate contaminated waste waters. � 2006 Elsevier Ltd. All rights reserved.",
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    author = "T Jong and David Parry",
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    Microbial sulfate reduction under sequentially acidic conditions in an upflow anaerobic packed bed bioreactor. / Jong, T; Parry, David.

    In: Water Research, Vol. 40, No. 13, 2006, p. 2561-2571.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Microbial sulfate reduction under sequentially acidic conditions in an upflow anaerobic packed bed bioreactor

    AU - Jong, T

    AU - Parry, David

    PY - 2006

    Y1 - 2006

    N2 - The aim of this study was to operate an upflow anaerobic packed bed reactor (UAPB) containing sulfate reducing bacteria (SRB) under acidic conditions similar to those found in acid mine drainage (AMD). The UAPB was filled with sand and operated under continuous flow at progressively lower pH and was shown to be capable of supporting sulfate reduction at pH values of 6.0, 5.0, 4.5, 4.0 and 3.5 in a synthetic medium containing 53.5 mmol l-1 lactate. Sulfate reduction rates of 553-1052 mmol m-3 d-1 were obtained when the influent solution pH was progressively lowered from pH 6.0 to 4.0, under an optimal flow rate of 2.61 ml min-1. When the influent pH was further lowered to pH 3.5, sulfate reduction was substantially reduced with only about 1% sulfate removed at a rate of 3.35 mmol m-3 d-1 after 20 days of operation. However, viable SRB were recovered from the column, indicating that the SRB population was capable of surviving and metabolizing at low levels even at pH 3.5 conditions for at least 20 days. The changes in conductivity in the SRB column did not always occur with changes in pH and redox potential, suggesting that conductivity measurements may be more sensitive to SRB activity and could be used as an additional tool for monitoring SRB activity. The bioreactor containing SRB was able to reduce sulfate and generate alkalinity even when challenged with influent as low as pH 3.5, indicating that such treatment systems have potential for bioremediating highly acidic, sulfate contaminated waste waters. � 2006 Elsevier Ltd. All rights reserved.

    AB - The aim of this study was to operate an upflow anaerobic packed bed reactor (UAPB) containing sulfate reducing bacteria (SRB) under acidic conditions similar to those found in acid mine drainage (AMD). The UAPB was filled with sand and operated under continuous flow at progressively lower pH and was shown to be capable of supporting sulfate reduction at pH values of 6.0, 5.0, 4.5, 4.0 and 3.5 in a synthetic medium containing 53.5 mmol l-1 lactate. Sulfate reduction rates of 553-1052 mmol m-3 d-1 were obtained when the influent solution pH was progressively lowered from pH 6.0 to 4.0, under an optimal flow rate of 2.61 ml min-1. When the influent pH was further lowered to pH 3.5, sulfate reduction was substantially reduced with only about 1% sulfate removed at a rate of 3.35 mmol m-3 d-1 after 20 days of operation. However, viable SRB were recovered from the column, indicating that the SRB population was capable of surviving and metabolizing at low levels even at pH 3.5 conditions for at least 20 days. The changes in conductivity in the SRB column did not always occur with changes in pH and redox potential, suggesting that conductivity measurements may be more sensitive to SRB activity and could be used as an additional tool for monitoring SRB activity. The bioreactor containing SRB was able to reduce sulfate and generate alkalinity even when challenged with influent as low as pH 3.5, indicating that such treatment systems have potential for bioremediating highly acidic, sulfate contaminated waste waters. � 2006 Elsevier Ltd. All rights reserved.

    KW - Conductivity

    KW - Microbial sulfate reduction

    KW - Sulfate reducing bacteria (SRB)

    KW - Upflow anaerobic packed bed reactor (UAPB)

    KW - Bioremediation

    KW - Metabolism

    KW - Packed beds

    KW - pH effects

    KW - Reduction

    KW - Wastewater treatment

    KW - Bioreactors

    KW - sulfate

    KW - acid mine drainage

    KW - bioreactor

    KW - bioremediation

    KW - conductivity

    KW - pH

    KW - redox potential

    KW - sulfate-reducing bacterium

    KW - wastewater

    KW - acidity

    KW - alkalinity

    KW - article

    KW - bacterial metabolism

    KW - bacterial survival

    KW - biotechnology

    KW - controlled study

    KW - flow rate

    KW - nonhuman

    KW - oxidation reduction reaction

    KW - priority journal

    KW - reduction kinetics

    KW - sulfate reducing bacterium

    KW - waste water management

    KW - Acids

    KW - Bacteria, Anaerobic

    KW - Biodegradation, Environmental

    KW - Conductometry

    KW - Efficiency

    KW - Lactic Acid

    KW - Oxidation-Reduction

    KW - Quality Control

    KW - Sulfates

    KW - Sulfides

    KW - Sulfur-Reducing Bacteria

    M3 - Article

    VL - 40

    SP - 2561

    EP - 2571

    JO - Water Research

    JF - Water Research

    SN - 0043-1354

    IS - 13

    ER -