@article{d168306485a34feab93a688864f52c3e,
title = "Microbiological Applications of High-Resolution Melting Analysis",
abstract = "High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis. ",
keywords = "adenine, cysteine, DNA, fluorescent dye, guanine, interferon, interleukin 28B, isoniazid, oseltamivir, quinoline derived antiinfective agent, rifampicin, sialidase, thymine, antibiotic resistance, antimalarial drug resistance, bacterial gene, Cryptococcus neoformans, DNA denaturation, DNA methylation, DNA sequence, Enterococcus faecium, financial management, genetic variability, genotype, gyrA gene, hepatitis C, high resolution melting analysis, influenza A (H1N1), katG gene, microbiological examination, multilocus sequence typing, Mycobacterium abscessus, Mycobacterium chelonei, neuraminidase gene, nonhuman, Plasmodium falciparum, priority journal, promoter region, real time polymerase chain reaction, rpoB gene, sensitivity and specificity, short survey, single nucleotide polymorphism, species identification, Staphylococcus aureus, tandem repeat, treatment response, tuberculosis, virus gene, Communicable Diseases, Microbiological Techniques, Molecular Diagnostic Techniques, Transition Temperature",
author = "Steven Tong and Philip Giffard",
year = "2012",
month = nov,
doi = "10.1128/JCM.01709-12",
language = "English",
volume = "50",
pages = "3418--3421",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",
}