Microbiological Applications of High-Resolution Melting Analysis

    Research output: Contribution to journalShort surveyResearchpeer-review

    Abstract

    High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.
    Original languageEnglish
    Pages (from-to)3418-3421
    Number of pages4
    JournalJournal of Clinical Microbiology
    Volume50
    Issue number11
    DOIs
    Publication statusPublished - Nov 2012

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    title = "Microbiological Applications of High-Resolution Melting Analysis",
    abstract = "High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.",
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    Microbiological Applications of High-Resolution Melting Analysis. / Tong, Steven; Giffard, Philip.

    In: Journal of Clinical Microbiology, Vol. 50, No. 11, 11.2012, p. 3418-3421.

    Research output: Contribution to journalShort surveyResearchpeer-review

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