Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)

Daniel J. Schmidt; Kathryn M. Real; David Crook; Jane Margaret Hughes

doi:10.1007/s12686-012-9800-9

Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)

Daniel J. Schmidt, Kathryn M. Real, David Crook, Jane Margaret Hughes

Research output: Contribution to journal › Article › peer-review

Abstract

Thirteen microsatellite loci were developed and characterised for two fishes from temperate Australia that exhibit atypical diadromous migration strategies. Cloning and sequencing of an enriched partial genomic library was used to develop seven highly polymorphic loci for the catadromous species Pseudaphritis urvillii (known as tupong or congolli). Mean number of alleles per locus was 16. 5, and average observed and expected heterozygosity was between 0. 90 and 0. 87, respectively. Six polymorphic markers characterised for the anadromous species Lovettia sealii (known as Tasmanian whitebait) included a mean of 12. 3 alleles per locus and average observed and expected heterozygosity of 0. 71-0. 77, respectively. These microsatellites will be employed to understand regional patterns of recruitment, migration and stock structure.

Original language	English
Pages (from-to)	347-349
Number of pages	3
Journal	Conservation Genetics Resources
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/s12686-012-9800-9
Publication status	Published - Jun 2013

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title = "Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)",

abstract = "Thirteen microsatellite loci were developed and characterised for two fishes from temperate Australia that exhibit atypical diadromous migration strategies. Cloning and sequencing of an enriched partial genomic library was used to develop seven highly polymorphic loci for the catadromous species Pseudaphritis urvillii (known as tupong or congolli). Mean number of alleles per locus was 16. 5, and average observed and expected heterozygosity was between 0. 90 and 0. 87, respectively. Six polymorphic markers characterised for the anadromous species Lovettia sealii (known as Tasmanian whitebait) included a mean of 12. 3 alleles per locus and average observed and expected heterozygosity of 0. 71-0. 77, respectively. These microsatellites will be employed to understand regional patterns of recruitment, migration and stock structure. ",

keywords = "Bovichthyidae, Galaxiidae, Lovettia sealii, Pisces, Pseudaphritis urvillii",

author = "Schmidt, {Daniel J.} and Real, {Kathryn M.} and David Crook and Hughes, {Jane Margaret}",

year = "2013",

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doi = "10.1007/s12686-012-9800-9",

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T1 - Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)

AU - Schmidt, Daniel J.

AU - Real, Kathryn M.

AU - Crook, David

AU - Hughes, Jane Margaret

PY - 2013/6

Y1 - 2013/6

N2 - Thirteen microsatellite loci were developed and characterised for two fishes from temperate Australia that exhibit atypical diadromous migration strategies. Cloning and sequencing of an enriched partial genomic library was used to develop seven highly polymorphic loci for the catadromous species Pseudaphritis urvillii (known as tupong or congolli). Mean number of alleles per locus was 16. 5, and average observed and expected heterozygosity was between 0. 90 and 0. 87, respectively. Six polymorphic markers characterised for the anadromous species Lovettia sealii (known as Tasmanian whitebait) included a mean of 12. 3 alleles per locus and average observed and expected heterozygosity of 0. 71-0. 77, respectively. These microsatellites will be employed to understand regional patterns of recruitment, migration and stock structure.

AB - Thirteen microsatellite loci were developed and characterised for two fishes from temperate Australia that exhibit atypical diadromous migration strategies. Cloning and sequencing of an enriched partial genomic library was used to develop seven highly polymorphic loci for the catadromous species Pseudaphritis urvillii (known as tupong or congolli). Mean number of alleles per locus was 16. 5, and average observed and expected heterozygosity was between 0. 90 and 0. 87, respectively. Six polymorphic markers characterised for the anadromous species Lovettia sealii (known as Tasmanian whitebait) included a mean of 12. 3 alleles per locus and average observed and expected heterozygosity of 0. 71-0. 77, respectively. These microsatellites will be employed to understand regional patterns of recruitment, migration and stock structure.

KW - Bovichthyidae

KW - Galaxiidae

KW - Lovettia sealii

KW - Pisces

KW - Pseudaphritis urvillii

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U2 - 10.1007/s12686-012-9800-9

DO - 10.1007/s12686-012-9800-9

M3 - Article

VL - 5

SP - 347

EP - 349

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -

Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)

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