Mitochondrial DNA supports the identification of two endangered river sharks (Glyphis glyphis and Glyphis garricki) across northern Australia

L WYNEN, H LARSON, D THORBURN, S PEVERELL, D MORGAN, Iain Field, Karen Gibb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The river sharks (genus Glyphis) are a small group of poorly known sharks occurring in tropical rivers and estuarine waters across northern Australia, south-east Asia and the subcontinent. The taxonomy of the genus has long been unclear due to very few individuals having been caught and examined, resulting in a paucity of data regarding their distribution, biology and ecology. Only recently has attention focussed on the two Australian species, G. glyphis and G. garricki. This study is a result of a rare opportunity to collate the few samples that have been collected from these species and the bull shark Carcharhinus leucas, which shares an overlapping range. These samples were analysed using the DNA barcoding approach (cox1 mitochondrial gene), compared with six other species of carcharhinids and evaluated in light of the current taxonomic classification. Nine species-specific nucleotide differences were found between G. glyphis and G. garricki and no intra-specific variation provides strong support for the separation into distinct species. Significant differences were also observed at the inter-generic level, with Glyphis forming a distinct clade from Carcharhinus. This study provides the basis for future molecular studies required to better address conservation issues confronting G. glyphis and G. garricki in Australia. � 2009 CSIRO.
    Original languageEnglish
    Pages (from-to)554-562
    Number of pages9
    JournalMarine and Freshwater Research
    Volume60
    Issue number6
    Publication statusPublished - 2009

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    Sharks
    shark
    sharks
    Mitochondrial DNA
    Rivers
    mitochondrial DNA
    Carcharhinus
    rivers
    river
    Leucas
    taxonomy
    South Australia
    Mitochondrial Genes
    Far East
    DNA barcoding
    river water
    Ecology
    brackish water
    South East Asia
    bulls

    Cite this

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    title = "Mitochondrial DNA supports the identification of two endangered river sharks (Glyphis glyphis and Glyphis garricki) across northern Australia",
    abstract = "The river sharks (genus Glyphis) are a small group of poorly known sharks occurring in tropical rivers and estuarine waters across northern Australia, south-east Asia and the subcontinent. The taxonomy of the genus has long been unclear due to very few individuals having been caught and examined, resulting in a paucity of data regarding their distribution, biology and ecology. Only recently has attention focussed on the two Australian species, G. glyphis and G. garricki. This study is a result of a rare opportunity to collate the few samples that have been collected from these species and the bull shark Carcharhinus leucas, which shares an overlapping range. These samples were analysed using the DNA barcoding approach (cox1 mitochondrial gene), compared with six other species of carcharhinids and evaluated in light of the current taxonomic classification. Nine species-specific nucleotide differences were found between G. glyphis and G. garricki and no intra-specific variation provides strong support for the separation into distinct species. Significant differences were also observed at the inter-generic level, with Glyphis forming a distinct clade from Carcharhinus. This study provides the basis for future molecular studies required to better address conservation issues confronting G. glyphis and G. garricki in Australia. � 2009 CSIRO.",
    keywords = "endangered species, estuarine ecosystem, genetic differentiation, mitochondrial DNA, shark, species conservation, taxonomy, tropical environment, Australasia, Australia, Carcharhinidae, Carcharhinus, Carcharhinus leucas, Chondrichthyes, Glyphis, Glyphis glyphis",
    author = "L WYNEN and H LARSON and D THORBURN and S PEVERELL and D MORGAN and Iain Field and Karen Gibb",
    year = "2009",
    language = "English",
    volume = "60",
    pages = "554--562",
    journal = "Marine & Freshwater Research",
    issn = "0067-1940",
    publisher = "CSIRO Publishing",
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    }

    Mitochondrial DNA supports the identification of two endangered river sharks (Glyphis glyphis and Glyphis garricki) across northern Australia. / WYNEN, L; LARSON, H; THORBURN, D; PEVERELL, S; MORGAN, D; Field, Iain; Gibb, Karen.

    In: Marine and Freshwater Research, Vol. 60, No. 6, 2009, p. 554-562.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Mitochondrial DNA supports the identification of two endangered river sharks (Glyphis glyphis and Glyphis garricki) across northern Australia

    AU - WYNEN, L

    AU - LARSON, H

    AU - THORBURN, D

    AU - PEVERELL, S

    AU - MORGAN, D

    AU - Field, Iain

    AU - Gibb, Karen

    PY - 2009

    Y1 - 2009

    N2 - The river sharks (genus Glyphis) are a small group of poorly known sharks occurring in tropical rivers and estuarine waters across northern Australia, south-east Asia and the subcontinent. The taxonomy of the genus has long been unclear due to very few individuals having been caught and examined, resulting in a paucity of data regarding their distribution, biology and ecology. Only recently has attention focussed on the two Australian species, G. glyphis and G. garricki. This study is a result of a rare opportunity to collate the few samples that have been collected from these species and the bull shark Carcharhinus leucas, which shares an overlapping range. These samples were analysed using the DNA barcoding approach (cox1 mitochondrial gene), compared with six other species of carcharhinids and evaluated in light of the current taxonomic classification. Nine species-specific nucleotide differences were found between G. glyphis and G. garricki and no intra-specific variation provides strong support for the separation into distinct species. Significant differences were also observed at the inter-generic level, with Glyphis forming a distinct clade from Carcharhinus. This study provides the basis for future molecular studies required to better address conservation issues confronting G. glyphis and G. garricki in Australia. � 2009 CSIRO.

    AB - The river sharks (genus Glyphis) are a small group of poorly known sharks occurring in tropical rivers and estuarine waters across northern Australia, south-east Asia and the subcontinent. The taxonomy of the genus has long been unclear due to very few individuals having been caught and examined, resulting in a paucity of data regarding their distribution, biology and ecology. Only recently has attention focussed on the two Australian species, G. glyphis and G. garricki. This study is a result of a rare opportunity to collate the few samples that have been collected from these species and the bull shark Carcharhinus leucas, which shares an overlapping range. These samples were analysed using the DNA barcoding approach (cox1 mitochondrial gene), compared with six other species of carcharhinids and evaluated in light of the current taxonomic classification. Nine species-specific nucleotide differences were found between G. glyphis and G. garricki and no intra-specific variation provides strong support for the separation into distinct species. Significant differences were also observed at the inter-generic level, with Glyphis forming a distinct clade from Carcharhinus. This study provides the basis for future molecular studies required to better address conservation issues confronting G. glyphis and G. garricki in Australia. � 2009 CSIRO.

    KW - endangered species

    KW - estuarine ecosystem

    KW - genetic differentiation

    KW - mitochondrial DNA

    KW - shark

    KW - species conservation

    KW - taxonomy

    KW - tropical environment

    KW - Australasia

    KW - Australia

    KW - Carcharhinidae

    KW - Carcharhinus

    KW - Carcharhinus leucas

    KW - Chondrichthyes

    KW - Glyphis

    KW - Glyphis glyphis

    M3 - Article

    VL - 60

    SP - 554

    EP - 562

    JO - Marine & Freshwater Research

    JF - Marine & Freshwater Research

    SN - 0067-1940

    IS - 6

    ER -