Molecular Characterization of High-Level-Cholera-Toxin-Producing El Tor Variant Vibrio cholerae Strains in the Zanzibar Archipelago of Tanzania

A Naha, G Chowdhury, J Ghosh-Banerjee, M Senoh, T Takahashi, BN Ley , Kamala Ley-Thriemer, Jaqueline Deen, Lorenz Von Seidlein, Said Mohammed Ali, Ahmed Khatib, T Ramamurthy, RK Nandy , G Balakrish Nair, Y Takeda, AK Mukhopadhyay

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.
Original languageEnglish
Pages (from-to)1040-1045
Number of pages6
JournalJournal of Clinical Microbiology
Volume51
Issue number3
DOIs
Publication statusPublished - 2013

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