Molecular Characterization of High-Level-Cholera-Toxin-Producing El Tor Variant Vibrio cholerae Strains in the Zanzibar Archipelago of Tanzania

A Naha, G Chowdhury, J Ghosh-Banerjee, M Senoh, T Takahashi, BN Ley , Kamala Ley-Thriemer, Jaqueline Deen, Lorenz Von Seidlein, Said Mohammed Ali, Ahmed Khatib, T Ramamurthy, RK Nandy , G Balakrish Nair, Y Takeda, AK Mukhopadhyay

    Research output: Contribution to journalArticle

    Abstract

    Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.
    Original languageEnglish
    Pages (from-to)1040-1045
    Number of pages6
    JournalJournal of Clinical Microbiology
    Volume51
    Issue number3
    DOIs
    Publication statusPublished - 2013

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