@article{476a2c465c9a450cafb1dfc4226e960d,
title = "Molecular Characterization of High-Level-Cholera-Toxin-Producing El Tor Variant Vibrio cholerae Strains in the Zanzibar Archipelago of Tanzania",
abstract = "Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis. ",
keywords = "cholera toxin, article, bacterial gene, bacterial strain, biotype, ctxB gene, feces analysis, genetic analysis, molecular genetics, nonhuman, nucleotide sequence, phenotype, polymerase chain reaction, priority journal, pulsed field gel electrophoresis, rstR gene, rtxC gene, strain difference, Tanzania, tcpA gene, toxin synthesis, Vibrio cholerae, Western blotting, Blotting, Western, Cholera, Cholera Toxin, Cluster Analysis, DNA, Bacterial, Electrophoresis, Gel, Pulsed-Field, Feces, Genotype, Humans, Molecular Sequence Data, Molecular Typing, Sequence Analysis, DNA, Vibrio cholerae O1",
author = "A Naha and G Chowdhury and J Ghosh-Banerjee and M Senoh and T Takahashi and BN Ley and Kamala Ley-Thriemer and Jaqueline Deen and {Von Seidlein}, Lorenz and Ali, {Said Mohammed} and Ahmed Khatib and T Ramamurthy and RK Nandy and Nair, {G Balakrish} and Y Takeda and AK Mukhopadhyay",
year = "2013",
doi = "10.1128/JCM.03162-12",
language = "English",
volume = "51",
pages = "1040--1045",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",
}