Molecular detection of the Australian grapevine yellows phytoplasma and comparison with grapevine yellows phytoplasmas from Italy

Anna Padovan, Karen Gibb, Assunta Bertaccini, M Vibio, Rob Bonfiglioli, Peter Magarey, Barbara Sears

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    A diagnostic test using the polymerase chain reaction is described for the detection of phytoplasma DNA in grapevines collected from South Australia and Victoria. Grapevines with Australian grapevine yellows disease tested positively for a phytoplasma but those with ‘restricted spring growth syndrome’ (formerly called ‘grapevine decline’) tested negatively. Restriction fragment length polymorphism analyses were done to determine the relationships between phytoplasmas of the Australian grapevine yellows and of representatives from both the aster yellows group (which includes phytoplasmas of grapevine yellows from Italy) and the elm yellows group (which includes phytoplasmas of flavescence dorée). Results showed that Australian grapevine yellows is associated with a unique phytoplasma that is more closely related to the phytoplasmas of the aster yellows group than to those of the elm yellows group.
    Original languageEnglish
    Pages (from-to)25-31
    Number of pages7
    JournalAustralian Journal of Grape and Wine Research
    Volume1
    Issue number1
    DOIs
    Publication statusPublished - Apr 1995

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    Australian grapevine yellows phytoplasma
    Phytoplasma
    Italy
    16SrV (Elm yellows group)
    16SrI (Aster yellows group)
    Victoria (Australia)
    South Australia
    diagnostic techniques
    restriction fragment length polymorphism
    polymerase chain reaction

    Cite this

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    abstract = "A diagnostic test using the polymerase chain reaction is described for the detection of phytoplasma DNA in grapevines collected from South Australia and Victoria. Grapevines with Australian grapevine yellows disease tested positively for a phytoplasma but those with ‘restricted spring growth syndrome’ (formerly called ‘grapevine decline’) tested negatively. Restriction fragment length polymorphism analyses were done to determine the relationships between phytoplasmas of the Australian grapevine yellows and of representatives from both the aster yellows group (which includes phytoplasmas of grapevine yellows from Italy) and the elm yellows group (which includes phytoplasmas of flavescence dor{\'e}e). Results showed that Australian grapevine yellows is associated with a unique phytoplasma that is more closely related to the phytoplasmas of the aster yellows group than to those of the elm yellows group.",
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    Molecular detection of the Australian grapevine yellows phytoplasma and comparison with grapevine yellows phytoplasmas from Italy. / Padovan, Anna; Gibb, Karen; Bertaccini, Assunta; Vibio, M; Bonfiglioli, Rob; Magarey, Peter; Sears, Barbara.

    In: Australian Journal of Grape and Wine Research, Vol. 1, No. 1, 04.1995, p. 25-31.

    Research output: Contribution to journalArticleResearchpeer-review

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    AU - Padovan, Anna

    AU - Gibb, Karen

    AU - Bertaccini, Assunta

    AU - Vibio, M

    AU - Bonfiglioli, Rob

    AU - Magarey, Peter

    AU - Sears, Barbara

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