Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians

MD Groves; B Crouch; Geoffrey Coombs; D Jordan; Stanley Pang; null Barton; Philip Giffard; Sam Abraham; null Trott

doi:10.1371/journal.pone.0146034

Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians

MD Groves, B Crouch, Geoffrey Coombs, D Jordan, Stanley Pang, Barton, Philip Giffard, Sam Abraham, Trott

Research output: Contribution to journal › Article › peer-review

16 Downloads (Pure)

Abstract

This work investigated the molecular epidemiology and antimicrobial resistance of methicillinresistant Staphylococcus aureus (MRSA) isolated from veterinarians in Australia in 2009. The collection (n = 44) was subjected to extensive molecular typing (MLST, spa, SCCmec, dru, PFGE, virulence and antimicrobial resistance genotyping) and antimicrobial resistance phenotyping by disk diffusion. MRSA was isolated from Australian veterinarians representing various occupational emphases. The isolate collection was dominated by MRSA strains belonging to clonal complex (CC) 8 and multilocus sequence type (ST) 22. CC8 MRSA (ST8-IV [2B], spa t064; and ST612-IV [2B], spa variable,) were strongly associated with equine practice veterinarians (OR = 17.5, 95% CI = 3.3-92.5, P < 0.001) and were often resistant to gentamicin and rifampicin. ST22-IV [2B], spa variable, were strongly associated with companion animal practice veterinarians (OR = 52.5, 95% CI = 5.2-532.7, P < 0.001) and were resistant to ciprofloxacin. A single pig practice veterinarian carried ST398-V [5C2], spa t1451. Equine practice and companion animal practice veterinarians frequently carried multiresistant-CC8 and ST22 MRSA, respectively, whereas only a single swine specialist carried MRSA ST398. The presence of these strains in veterinarians may be associated with specific antimicrobial administration practices in each animal species.

Original language	English
Article number	e0146034
Pages (from-to)	1-12
Number of pages	12
Journal	PLoS One
Volume	11
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0146034
Publication status	Published - 2016

Access to Document

10.1371/journal.pone.0146034Licence: CC BY

5294728-oaFinal published version, 243 KBLicence: CC BY

Cite this

@article{df2d0044ddff4b01a6c2836b6b5aa348,

title = "Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians",

abstract = "This work investigated the molecular epidemiology and antimicrobial resistance of methicillinresistant Staphylococcus aureus (MRSA) isolated from veterinarians in Australia in 2009. The collection (n = 44) was subjected to extensive molecular typing (MLST, spa, SCCmec, dru, PFGE, virulence and antimicrobial resistance genotyping) and antimicrobial resistance phenotyping by disk diffusion. MRSA was isolated from Australian veterinarians representing various occupational emphases. The isolate collection was dominated by MRSA strains belonging to clonal complex (CC) 8 and multilocus sequence type (ST) 22. CC8 MRSA (ST8-IV [2B], spa t064; and ST612-IV [2B], spa variable,) were strongly associated with equine practice veterinarians (OR = 17.5, 95% CI = 3.3-92.5, P < 0.001) and were often resistant to gentamicin and rifampicin. ST22-IV [2B], spa variable, were strongly associated with companion animal practice veterinarians (OR = 52.5, 95% CI = 5.2-532.7, P < 0.001) and were resistant to ciprofloxacin. A single pig practice veterinarian carried ST398-V [5C2], spa t1451. Equine practice and companion animal practice veterinarians frequently carried multiresistant-CC8 and ST22 MRSA, respectively, whereas only a single swine specialist carried MRSA ST398. The presence of these strains in veterinarians may be associated with specific antimicrobial administration practices in each animal species. ",

keywords = "ciprofloxacin, clindamycin, gentamicin, rifampicin, tetracycline, trimethoprim, Article, Australian, bacterial gene, bacterium isolation, disk diffusion, gene cassette, gene identification, genotype phenotype correlation, human, mecA gene, methicillin susceptible Staphylococcus aureus, molecular epidemiology, multilocus sequence typing, nonhuman, nuc gene, occupational exposure, prevalence, pulsed field gel electrophoresis, spa gene, veterinarian, veterinary medicine",

author = "MD Groves and B Crouch and Geoffrey Coombs and D Jordan and Stanley Pang and Barton and Philip Giffard and Sam Abraham and Trott",

note = "Australian Research Council grant LP13010073",

year = "2016",

doi = "10.1371/journal.pone.0146034",

language = "English",

volume = "11",

pages = "1--12",

journal = "PLoS One",

issn = "1932-6203",

publisher = "Public Library of Science (PLoS)",

number = "1",

}

TY - JOUR

T1 - Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians

AU - Groves, MD

AU - Crouch, B

AU - Coombs, Geoffrey

AU - Jordan, D

AU - Pang, Stanley

AU - Barton, null

AU - Giffard, Philip

AU - Abraham, Sam

AU - Trott, null

N1 - Australian Research Council grant LP13010073

PY - 2016

Y1 - 2016

N2 - This work investigated the molecular epidemiology and antimicrobial resistance of methicillinresistant Staphylococcus aureus (MRSA) isolated from veterinarians in Australia in 2009. The collection (n = 44) was subjected to extensive molecular typing (MLST, spa, SCCmec, dru, PFGE, virulence and antimicrobial resistance genotyping) and antimicrobial resistance phenotyping by disk diffusion. MRSA was isolated from Australian veterinarians representing various occupational emphases. The isolate collection was dominated by MRSA strains belonging to clonal complex (CC) 8 and multilocus sequence type (ST) 22. CC8 MRSA (ST8-IV [2B], spa t064; and ST612-IV [2B], spa variable,) were strongly associated with equine practice veterinarians (OR = 17.5, 95% CI = 3.3-92.5, P < 0.001) and were often resistant to gentamicin and rifampicin. ST22-IV [2B], spa variable, were strongly associated with companion animal practice veterinarians (OR = 52.5, 95% CI = 5.2-532.7, P < 0.001) and were resistant to ciprofloxacin. A single pig practice veterinarian carried ST398-V [5C2], spa t1451. Equine practice and companion animal practice veterinarians frequently carried multiresistant-CC8 and ST22 MRSA, respectively, whereas only a single swine specialist carried MRSA ST398. The presence of these strains in veterinarians may be associated with specific antimicrobial administration practices in each animal species.

AB - This work investigated the molecular epidemiology and antimicrobial resistance of methicillinresistant Staphylococcus aureus (MRSA) isolated from veterinarians in Australia in 2009. The collection (n = 44) was subjected to extensive molecular typing (MLST, spa, SCCmec, dru, PFGE, virulence and antimicrobial resistance genotyping) and antimicrobial resistance phenotyping by disk diffusion. MRSA was isolated from Australian veterinarians representing various occupational emphases. The isolate collection was dominated by MRSA strains belonging to clonal complex (CC) 8 and multilocus sequence type (ST) 22. CC8 MRSA (ST8-IV [2B], spa t064; and ST612-IV [2B], spa variable,) were strongly associated with equine practice veterinarians (OR = 17.5, 95% CI = 3.3-92.5, P < 0.001) and were often resistant to gentamicin and rifampicin. ST22-IV [2B], spa variable, were strongly associated with companion animal practice veterinarians (OR = 52.5, 95% CI = 5.2-532.7, P < 0.001) and were resistant to ciprofloxacin. A single pig practice veterinarian carried ST398-V [5C2], spa t1451. Equine practice and companion animal practice veterinarians frequently carried multiresistant-CC8 and ST22 MRSA, respectively, whereas only a single swine specialist carried MRSA ST398. The presence of these strains in veterinarians may be associated with specific antimicrobial administration practices in each animal species.

KW - ciprofloxacin

KW - clindamycin

KW - gentamicin

KW - rifampicin

KW - tetracycline

KW - trimethoprim

KW - Article

KW - Australian

KW - bacterial gene

KW - bacterium isolation

KW - disk diffusion

KW - gene cassette

KW - gene identification

KW - genotype phenotype correlation

KW - human

KW - mecA gene

KW - methicillin susceptible Staphylococcus aureus

KW - molecular epidemiology

KW - multilocus sequence typing

KW - nonhuman

KW - nuc gene

KW - occupational exposure

KW - prevalence

KW - pulsed field gel electrophoresis

KW - spa gene

KW - veterinarian

KW - veterinary medicine

UR - http://www.scopus.com/inward/record.url?scp=84954149670&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0146034

DO - 10.1371/journal.pone.0146034

M3 - Article

C2 - 26735694

VL - 11

SP - 1

EP - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e0146034

ER -

Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from Australian veterinarians

Abstract

Access to Document

Other files and links

Fingerprint

Cite this