Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE)

Tuty Arisuryanti; Nu Wei Vivian Wei; Chris Austin

doi:10.1063/1.4953534

Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE)

Tuty Arisuryanti, Nu Wei Vivian Wei, Chris Austin

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper published in Proceedings › peer-review

Abstract

Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.

Original language	English
Title of host publication	Proceeding of the 4th International Conference on Biological Science, ICBS 2015
Subtitle of host publication	Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research
Editors	R.H. Setyobudi, T.R. Nuringtyas, P.G. Adinurani
Publisher	American Institute of Physics
Pages	1-6
Number of pages	6
Volume	1744
Edition	Issue 1
ISBN (Electronic)	9780735414013
DOIs	https://doi.org/10.1063/1.4953534
Publication status	Published - 14 Jun 2016
Event	4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015 - Yogyakarta, Indonesia Duration: 18 Sep 2015 → 19 Sep 2015

Conference

Conference	4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015
Country/Territory	Indonesia
City	Yogyakarta
Period	18/09/15 → 19/09/15

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10.1063/1.4953534

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Arisuryanti, T., Wei, N. W. V., & Austin, C. (2016). Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). In R. H. Setyobudi, T. R. Nuringtyas, & P. G. Adinurani (Eds.), Proceeding of the 4th International Conference on Biological Science, ICBS 2015: Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research (Issue 1 ed., Vol. 1744, pp. 1-6). [020060] American Institute of Physics. https://doi.org/10.1063/1.4953534

Arisuryanti, Tuty ; Wei, Nu Wei Vivian ; Austin, Chris. / Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). Proceeding of the 4th International Conference on Biological Science, ICBS 2015: Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research. editor / R.H. Setyobudi ; T.R. Nuringtyas ; P.G. Adinurani. Vol. 1744 Issue 1. ed. American Institute of Physics, 2016. pp. 1-6

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abstract = "Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.",

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Arisuryanti, T, Wei, NWV & Austin, C 2016, Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). in RH Setyobudi, TR Nuringtyas & PG Adinurani (eds), Proceeding of the 4th International Conference on Biological Science, ICBS 2015: Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research. Issue 1 edn, vol. 1744, 020060, American Institute of Physics, pp. 1-6, 4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015, Yogyakarta, Indonesia, 18/09/15. https://doi.org/10.1063/1.4953534

Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). / Arisuryanti, Tuty; Wei, Nu Wei Vivian; Austin, Chris.

Proceeding of the 4th International Conference on Biological Science, ICBS 2015: Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research. ed. / R.H. Setyobudi; T.R. Nuringtyas; P.G. Adinurani. Vol. 1744 Issue 1. ed. American Institute of Physics, 2016. p. 1-6 020060.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper published in Proceedings › peer-review

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N2 - Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.

AB - Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.

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Arisuryanti T, Wei NWV, Austin C. Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). In Setyobudi RH, Nuringtyas TR, Adinurani PG, editors, Proceeding of the 4th International Conference on Biological Science, ICBS 2015: Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research. Issue 1 ed. Vol. 1744. American Institute of Physics. 2016. p. 1-6. 020060 https://doi.org/10.1063/1.4953534

Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE)

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