Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE)

Tuty Arisuryanti, Nu Wei Vivian Wei, Chris Austin

    Research output: Chapter in Book/Report/Conference proceedingConference Paper published in Proceedings

    Abstract

    Swamp eel is the Indonesian freshwater fish that should be understood and preserved due to their high economic potency in aquaculture. Additionally Indonesian swamp eel is commonly accepted to the species described as Monopterus albus (Synbranchiformes: Synbranchidae). However, the knowledge of genetic variation of this species from Indonesia is quite limited. Hence, in this study, we used the Denaturing Gradient Gel Electrophoresis (DGGE) and surveyed the genetic variation of cytochrome c oxidase subunit I (COI) in Indonesian swamp eels. Total of 300 samples of the swamp eel fish were collected from 15 populations in Indonesia. The DGGE results represented two genetic patterns (A and B) of the COI variations. In addition, the results revealed that five populations showed pattern A and six populations exhibited pattern B while four populations showed both patterns A and B of the COI variations. Therefore, the result supported the molecular data that the Indonesian swamp eel is a cryptic species complex. In addition, the distribution of swamp eel's COI patterns in Indonesia approves that DGGE is a rapid and sensitive method for the detection of a genetic variation of the fish.

    Original languageEnglish
    Title of host publicationTowards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research - Proceeding of the 4th International Conference on Biological Science, ICBS 2015
    PublisherAmerican Institute of Physics
    Volume1744
    EditionIssue 1
    ISBN (Electronic)9780735414013
    DOIs
    Publication statusPublished - 14 Jun 2016
    Event4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015 - Yogyakarta, Indonesia
    Duration: 18 Sep 201519 Sep 2015

    Conference

    Conference4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015
    CountryIndonesia
    CityYogyakarta
    Period18/09/1519/09/15

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    Arisuryanti, T., Wei, N. W. V., & Austin, C. (2016). Molecular evidence for determination cryptic species of Indonesian swamp eel populations using denaturing gradient gel electrophoresis (DGGE). In Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research - Proceeding of the 4th International Conference on Biological Science, ICBS 2015 (Issue 1 ed., Vol. 1744). [020060] American Institute of Physics. https://doi.org/10.1063/1.4953534