Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast

Jean Pierre Hermand, Longxiang Guo, Joanne Randall, Patrice Brehmer

Research output: Chapter in Book/Report/Conference proceedingConference Paper published in ProceedingsResearchpeer-review

Abstract

A submersible digital holographic microscope (DHM) Holoflow@Sea was deployed during AWA fisheries surveys conducted in March 2013 and 2014 in the large upwelling ecosystem of the Canaries Current. The microscope collected about half a million of holograms of microorganisms and other particles suspended in the water column. Selected new results of reconstruction of phyto- and zooplankton species using improved algorithms with respect to previously published results are presented. Amplitude images are more detailed and, for some species, phase images provide useful additional information about morphology and composition. Extensive analysis confirms that the submersible DHM is suitable for in-situ imaging of organisms in the size range 2 μm-200 μm and species identification. When compared to standard procedures the microscope allows to rapidly monitor planktonic communities over a wide area. It contributes to a better understanding of the organization and functioning of the ecosystem and in particular the link between biogeochemistry cycles and small pelagic fish stock dynamics.

Original languageEnglish
Title of host publicationOCEANS 2014 - TAIPEI
PublisherIEEE, Institute of Electrical and Electronics Engineers
Number of pages5
ISBN (Electronic)9781479936465
DOIs
Publication statusPublished - 24 Nov 2014
Externally publishedYes
EventOCEANS 2014 MTS/IEEE Taipei Conference: Oceans Regeneration - Taipei, Taiwan, Province of China
Duration: 7 Apr 201410 Apr 2014

Conference

ConferenceOCEANS 2014 MTS/IEEE Taipei Conference: Oceans Regeneration
CountryTaiwan, Province of China
CityTaipei
Period7/04/1410/04/14

Fingerprint

Microorganisms
Coastal zones
Microscopes
Imaging techniques
Ecosystems
Biogeochemistry
Fisheries
Phytoplankton
Holograms
Fish
Chemical analysis
Water

Cite this

Hermand, J. P., Guo, L., Randall, J., & Brehmer, P. (2014). Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast. In OCEANS 2014 - TAIPEI [6964569] IEEE, Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/OCEANS-TAIPEI.2014.6964569
Hermand, Jean Pierre ; Guo, Longxiang ; Randall, Joanne ; Brehmer, Patrice. / Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast. OCEANS 2014 - TAIPEI. IEEE, Institute of Electrical and Electronics Engineers, 2014.
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Hermand, JP, Guo, L, Randall, J & Brehmer, P 2014, Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast. in OCEANS 2014 - TAIPEI., 6964569, IEEE, Institute of Electrical and Electronics Engineers, OCEANS 2014 MTS/IEEE Taipei Conference: Oceans Regeneration, Taipei, Taiwan, Province of China, 7/04/14. https://doi.org/10.1109/OCEANS-TAIPEI.2014.6964569

Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast. / Hermand, Jean Pierre; Guo, Longxiang; Randall, Joanne; Brehmer, Patrice.

OCEANS 2014 - TAIPEI. IEEE, Institute of Electrical and Electronics Engineers, 2014. 6964569.

Research output: Chapter in Book/Report/Conference proceedingConference Paper published in ProceedingsResearchpeer-review

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Hermand JP, Guo L, Randall J, Brehmer P. Non-destructive optical holographic imaging of microorganisms in situ off the Senegalese coast. In OCEANS 2014 - TAIPEI. IEEE, Institute of Electrical and Electronics Engineers. 2014. 6964569 https://doi.org/10.1109/OCEANS-TAIPEI.2014.6964569