Non-typeable H. influenzae-specific immune responses in children with chronic suppurative lung disease

Susan  J. Pizzutto; Stephanie Yerkovich; Belinda J. Hales; Wayne Thomas; John W. Upham; Anne Chang

doi:10.1111/resp.12183_31

Non-typeable H. influenzae-specific immune responses in children with chronic suppurative lung disease

Susan J. Pizzutto, Stephanie Yerkovich, Belinda J. Hales, Wayne Thomas, John W. Upham, Anne Chang

Child and Maternal Health

Research output: Contribution to journal › Comment/debate

Abstract

Introduction: Non-typeable H. influenzae(NTHi) is the most common bacterial pathogen associated with chronic suppurative lung disease (CSLD). Limited data exists regarding the adaptive immune response to NTHi and the role it may have in the aetiology of CSLD in children.

Aim: To determine if children with CSLD have a suboptimal adaptive immune response to NTHi.

Method: NTHi-stimulated cytokine (IFNg, IL-13 and IL-10) production from peripheral blood mononuclear cells (PBMC) were measured in 82 children with CSLD and 51 healthy control children (HC). Plasma antibody titres (IgG1,IgG4) to the H. Influenzae outer membrane proteins P4 and P6 were also measured.

Results: Compared to healthy controls, PBMC from children with CSLD pro-duced significantly more IL-13 (p=0.030) and significantly less IFNg(p<0.001) and IL-10 (p=0.029) in response to NTHi. CSLD was associated with significantly lower P4-specific IgG1 titres but there was no significant difference in P6-specific IgG1 between the two groups. The prevalence of detectable IgG4 to both P4 and P6 was low in both groups however the level of response to P6 was significantly lower in children with CSLD (p<0.001).IL-10 was positively correlated with P4 and P6-specific IgG4 (p=0.022 and p=0.003 respectively).

Conclusion: Cell mediated immunity, in particular the IFNg response, likely plays an important role in protective immunity against NTHi in children. The inability to elicit a strong IFNg response to NTHi may contribute to the patho-genesis of CSLD. Whilst the cell-mediated immune response in CSLD isTH2-skewed (high IL-13, low IFNg), low IL-10 production and low IgG4 titres suggest a more complex form of immune dysregulation in children with CSLD.

Original language	English
Article number	OS183
Pages (from-to)	59-59
Number of pages	1
Journal	Respirology
Volume	18
Issue number	S4
DOIs	https://doi.org/10.1111/resp.12183_31
Publication status	Published - Nov 2013
Event	Congress of the Asian Pacific Society of Respirology - Conference Center Pacifico Yokohama, Yokohama, Japan Duration: 11 Nov 2013 → 14 Nov 2013 Conference number: 18th

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10.1111/resp.12183_31

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@article{b900674cf9ac4e53b03fed4944168144,

title = "Non-typeable H. influenzae-specific immune responses in children with chronic suppurative lung disease",

abstract = "Introduction: Non-typeable H. influenzae(NTHi) is the most common bacterial pathogen associated with chronic suppurative lung disease (CSLD). Limited data exists regarding the adaptive immune response to NTHi and the role it may have in the aetiology of CSLD in children. Aim: To determine if children with CSLD have a suboptimal adaptive immune response to NTHi. Method: NTHi-stimulated cytokine (IFNg, IL-13 and IL-10) production from peripheral blood mononuclear cells (PBMC) were measured in 82 children with CSLD and 51 healthy control children (HC). Plasma antibody titres (IgG1,IgG4) to the H. Influenzae outer membrane proteins P4 and P6 were also measured. Results: Compared to healthy controls, PBMC from children with CSLD pro-duced significantly more IL-13 (p=0.030) and significantly less IFNg(p<0.001) and IL-10 (p=0.029) in response to NTHi. CSLD was associated with significantly lower P4-specific IgG1 titres but there was no significant difference in P6-specific IgG1 between the two groups. The prevalence of detectable IgG4 to both P4 and P6 was low in both groups however the level of response to P6 was significantly lower in children with CSLD (p<0.001).IL-10 was positively correlated with P4 and P6-specific IgG4 (p=0.022 and p=0.003 respectively). Conclusion: Cell mediated immunity, in particular the IFNg response, likely plays an important role in protective immunity against NTHi in children. The inability to elicit a strong IFNg response to NTHi may contribute to the patho-genesis of CSLD. Whilst the cell-mediated immune response in CSLD isTH2-skewed (high IL-13, low IFNg), low IL-10 production and low IgG4 titres suggest a more complex form of immune dysregulation in children with CSLD.",

author = "{J. Pizzutto}, Susan and Stephanie Yerkovich and Hales, {Belinda J.} and Wayne Thomas and Upham, {John W.} and Anne Chang",

year = "2013",

month = nov,

doi = "10.1111/resp.12183_31",

language = "English",

volume = "18",

pages = "59--59",

journal = "Respirology",

issn = "1323-7799",

publisher = "Wiley-Blackwell",

number = "S4",

note = "Congress of the Asian Pacific Society of Respirology ; Conference date: 11-11-2013 Through 14-11-2013",

}

TY - JOUR

T1 - Non-typeable H. influenzae-specific immune responses in children with chronic suppurative lung disease

AU - J. Pizzutto, Susan

AU - Yerkovich, Stephanie

AU - Hales, Belinda J.

AU - Thomas, Wayne

AU - Upham, John W.

AU - Chang, Anne

N1 - Conference code: 18th

PY - 2013/11

Y1 - 2013/11

N2 - Introduction: Non-typeable H. influenzae(NTHi) is the most common bacterial pathogen associated with chronic suppurative lung disease (CSLD). Limited data exists regarding the adaptive immune response to NTHi and the role it may have in the aetiology of CSLD in children. Aim: To determine if children with CSLD have a suboptimal adaptive immune response to NTHi. Method: NTHi-stimulated cytokine (IFNg, IL-13 and IL-10) production from peripheral blood mononuclear cells (PBMC) were measured in 82 children with CSLD and 51 healthy control children (HC). Plasma antibody titres (IgG1,IgG4) to the H. Influenzae outer membrane proteins P4 and P6 were also measured. Results: Compared to healthy controls, PBMC from children with CSLD pro-duced significantly more IL-13 (p=0.030) and significantly less IFNg(p<0.001) and IL-10 (p=0.029) in response to NTHi. CSLD was associated with significantly lower P4-specific IgG1 titres but there was no significant difference in P6-specific IgG1 between the two groups. The prevalence of detectable IgG4 to both P4 and P6 was low in both groups however the level of response to P6 was significantly lower in children with CSLD (p<0.001).IL-10 was positively correlated with P4 and P6-specific IgG4 (p=0.022 and p=0.003 respectively). Conclusion: Cell mediated immunity, in particular the IFNg response, likely plays an important role in protective immunity against NTHi in children. The inability to elicit a strong IFNg response to NTHi may contribute to the patho-genesis of CSLD. Whilst the cell-mediated immune response in CSLD isTH2-skewed (high IL-13, low IFNg), low IL-10 production and low IgG4 titres suggest a more complex form of immune dysregulation in children with CSLD.

AB - Introduction: Non-typeable H. influenzae(NTHi) is the most common bacterial pathogen associated with chronic suppurative lung disease (CSLD). Limited data exists regarding the adaptive immune response to NTHi and the role it may have in the aetiology of CSLD in children. Aim: To determine if children with CSLD have a suboptimal adaptive immune response to NTHi. Method: NTHi-stimulated cytokine (IFNg, IL-13 and IL-10) production from peripheral blood mononuclear cells (PBMC) were measured in 82 children with CSLD and 51 healthy control children (HC). Plasma antibody titres (IgG1,IgG4) to the H. Influenzae outer membrane proteins P4 and P6 were also measured. Results: Compared to healthy controls, PBMC from children with CSLD pro-duced significantly more IL-13 (p=0.030) and significantly less IFNg(p<0.001) and IL-10 (p=0.029) in response to NTHi. CSLD was associated with significantly lower P4-specific IgG1 titres but there was no significant difference in P6-specific IgG1 between the two groups. The prevalence of detectable IgG4 to both P4 and P6 was low in both groups however the level of response to P6 was significantly lower in children with CSLD (p<0.001).IL-10 was positively correlated with P4 and P6-specific IgG4 (p=0.022 and p=0.003 respectively). Conclusion: Cell mediated immunity, in particular the IFNg response, likely plays an important role in protective immunity against NTHi in children. The inability to elicit a strong IFNg response to NTHi may contribute to the patho-genesis of CSLD. Whilst the cell-mediated immune response in CSLD isTH2-skewed (high IL-13, low IFNg), low IL-10 production and low IgG4 titres suggest a more complex form of immune dysregulation in children with CSLD.

U2 - 10.1111/resp.12183_31

DO - 10.1111/resp.12183_31

M3 - Comment/debate

SN - 1323-7799

VL - 18

SP - 59

EP - 59

JO - Respirology

JF - Respirology

IS - S4

M1 - OS183

T2 - Congress of the Asian Pacific Society of Respirology

Y2 - 11 November 2013 through 14 November 2013

ER -

Non-typeable H. influenzae-specific immune responses in children with chronic suppurative lung disease

Abstract

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