TY - JOUR
T1 - Novel Catabolic Pathway of Quercetin-3-O-Rutinose-7-O-α-L-Rhamnoside by Lactobacillus plantarum GDMCC 1.140
T2 - The Direct Fission of C-Ring
AU - Huang, Guitao
AU - Lai, Mingwen
AU - Xu, Canhua
AU - He, Shan
AU - Dong, Lihong
AU - Huang, Fei
AU - Zhang, Ruifen
AU - Young, David James
AU - Liu, Hesheng
AU - Su, Dongxiao
N1 - Funding Information:
This study was supported by the Zhejiang Provincial Top Discipline of Biological Engineering (Grant No. KF2021005), the Science and Technology Program of Guangzhou (Grant No. 202102010451), the Guangdong Province's Key Research and Development Program (Grant No. 2020B0202080003), and the Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences (Grant No. 202108TD).
Publisher Copyright:
Copyright © 2022 Huang, Lai, Xu, He, Dong, Huang, Zhang, Young, Liu and Su.
PY - 2022/3/16
Y1 - 2022/3/16
N2 - Lychee pulp phenolics (LPP) is mainly catabolized in the host colon, increasing the abundances of Bacteroides and Lactobacillus. Herein, five selected gut microbial strains (Bacteroides uniformis, B. thetaiotaomicron, Lactobacillus rhamnosus, L. plantarum, and L. acidophilus) were separately incubated with LPP to ascertain the specific strains participating in phenolic metabolism and the corresponding metabolites. The results indicated that B. uniformis, L. rhamnosus, and L. plantarum were involved in LPP utilization, contributing to 52.37, 28.33, and 45.11% of LPP degradation after 48 h fermentation, respectively. Unprecedentedly, the metabolic pathway of the major phenolic compound quercetin-3-O-rutinose-7-O-α-L-rhamnoside by L. plantarum, appeared to be the direct fission of C-ring at C2–O1 and C3–C4 bonds, which was proved from the occurrence of two substances with the deprotonated molecule [M–H]− ion at m/z 299 and 459, respectively. Meanwhile, it was fully confirmed that B. uniformis participated in the catabolism of isorhamnetin glycoside and procyanidin B2. In the B. uniformis culture, kaempferol was synthesized through dehydroxylation of quercetin which could be catabolized into alphitonin by L. rhamnosus. Furthermore, LPP metabolites exerted higher antioxidant activity than their precursors and gave clues to understand the interindividual differences for phenolic metabolism by gut microbiota.
AB - Lychee pulp phenolics (LPP) is mainly catabolized in the host colon, increasing the abundances of Bacteroides and Lactobacillus. Herein, five selected gut microbial strains (Bacteroides uniformis, B. thetaiotaomicron, Lactobacillus rhamnosus, L. plantarum, and L. acidophilus) were separately incubated with LPP to ascertain the specific strains participating in phenolic metabolism and the corresponding metabolites. The results indicated that B. uniformis, L. rhamnosus, and L. plantarum were involved in LPP utilization, contributing to 52.37, 28.33, and 45.11% of LPP degradation after 48 h fermentation, respectively. Unprecedentedly, the metabolic pathway of the major phenolic compound quercetin-3-O-rutinose-7-O-α-L-rhamnoside by L. plantarum, appeared to be the direct fission of C-ring at C2–O1 and C3–C4 bonds, which was proved from the occurrence of two substances with the deprotonated molecule [M–H]− ion at m/z 299 and 459, respectively. Meanwhile, it was fully confirmed that B. uniformis participated in the catabolism of isorhamnetin glycoside and procyanidin B2. In the B. uniformis culture, kaempferol was synthesized through dehydroxylation of quercetin which could be catabolized into alphitonin by L. rhamnosus. Furthermore, LPP metabolites exerted higher antioxidant activity than their precursors and gave clues to understand the interindividual differences for phenolic metabolism by gut microbiota.
KW - Bacteroides uniformis
KW - C-ring fission
KW - gut microbiota
KW - Lactobacillus
KW - lychee
UR - http://www.scopus.com/inward/record.url?scp=85127737656&partnerID=8YFLogxK
U2 - 10.3389/fnut.2022.849439
DO - 10.3389/fnut.2022.849439
M3 - Article
C2 - 35369057
AN - SCOPUS:85127737656
VL - 9
SP - 1
EP - 14
JO - Frontiers in Nutrition
JF - Frontiers in Nutrition
SN - 2296-861X
M1 - 849439
ER -