Optimisation of Calophyllum inophyllum seed oil nanoemulsion as a potential wound healing agent

Elnaz Saki, Vinuthaa Murthy, Roshanak Khandanlou, Hao Wang, Johanna Wapling, Richard P. Weir

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Background: Efficient delivery systems of Calophyllum inophyllum seed oil (CSO) in the form of nanoemulsion were optimised to enhance its stability and ensure its therapeutic efficiency as a potential agent for various biomedical applications.

Method: Response Surface Methodology (RSM) was used to determine the effects of independent variables (oil, surfactant, water percentage and homogenisation time) on physicochemical characteristics, including droplet size, polydispersity index and turbidity.

Results: The optimised CSO nanoemulsion (CSONE) has a 46.68 nm particle size, 0.15 Polydispersity index value and 1.16 turbidity. After 4 weeks of storage at 5 ± 1 °C and 25 ± 1 °C, the CSONE was physically stable. The optimised CSO nanoemulsion showed enhancement in cell viability and wound healing in baby hamster kidney a clone BHK-21 (BSR) cells as compared to the CSO. The wound healing property of CSONE was higher than CSO.

Conclusion: Thus, our in vitro wound healing results demonstrated that CSO in the nanoemulsion form can promote wound healing by enhancing the proliferation and migration of epidermal cells.
Original languageEnglish
Article number285
Pages (from-to)1-15
Number of pages15
JournalBMC Complementary Medicine and Therapies
Issue number285
Publication statusPublished - Dec 2022


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