Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei

first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]

David Dance, Vanaporn Wuthiekanun, Derek Sarovich, Erin Price, Direk Limmathurotsakul, Bart Currie, Trinh Thanh Trung

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    Abstract

    We are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least.
    Original languageEnglish
    Pages (from-to)117-118
    Number of pages2
    JournalInternational Medical Case Reports Journal
    Volume12
    DOIs
    Publication statusPublished - 18 Apr 2019

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    Melioidosis
    Burkholderia pseudomallei
    Indonesia
    Biofilms
    Pharmaceutical Preparations
    Burkholderia
    Multilocus Sequence Typing
    Oxidoreductases
    Research Personnel
    Polymerase Chain Reaction
    Genes

    Cite this

    Dance, David ; Wuthiekanun, Vanaporn ; Sarovich, Derek ; Price, Erin ; Limmathurotsakul, Direk ; Currie, Bart ; Trung, Trinh Thanh. / Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei : first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]. In: International Medical Case Reports Journal. 2019 ; Vol. 12. pp. 117-118.
    @article{295d31a7c3b14d90a12e8df375b17661,
    title = "Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]",
    abstract = "We are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least.",
    author = "David Dance and Vanaporn Wuthiekanun and Derek Sarovich and Erin Price and Direk Limmathurotsakul and Bart Currie and Trung, {Trinh Thanh}",
    year = "2019",
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    pages = "117--118",
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    Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei : first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]. / Dance, David; Wuthiekanun, Vanaporn; Sarovich, Derek; Price, Erin; Limmathurotsakul, Direk; Currie, Bart; Trung, Trinh Thanh.

    In: International Medical Case Reports Journal, Vol. 12, 18.04.2019, p. 117-118.

    Research output: Contribution to journalLetterResearchpeer-review

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    T1 - Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei

    T2 - first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]

    AU - Dance, David

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    AU - Price, Erin

    AU - Limmathurotsakul, Direk

    AU - Currie, Bart

    AU - Trung, Trinh Thanh

    PY - 2019/4/18

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    N2 - We are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least.

    AB - We are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least.

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