Pervasive admixture between eucalypt species has consequences for conservation and assisted migration

Brenton von Takach Dukai, Cameron Jack, Justin Borevitz, David B. Lindenmayer, Sam C. Banks

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    Abstract

    Conservation management often uses information on genetic population structure to assess the importance of local provenancing for ecological restoration and reintroduction programs. For species that do not exhibit complete reproductive isolation, the estimation of population genetic parameters may be influenced by the extent of admixture. Therefore, to avoid perverse outcomes for conservation, genetically informed management strategies must determine whether hybridization between species is relevant, and the extent to which observed population genetic patterns are shaped by interspecific versus intraspecific gene flow. We used genotyping by sequencing to identify over 2,400 informative single nucleotide polymorphisms across 18 populations of Eucalyptus regnans F. Muell., a foundation tree species of montane forests in south-eastern Australia. We used these data to determine the extent of hybridization with another species, Eucalyptus obliqua L'Hér., and investigate how admixture influences genetic diversity parameters, by estimating metrics of genetic diversity and examining population genetic structure in datasets with and without admixed individuals. We found hybrid individuals at all sites and two highly introgressed populations. Hybrid individuals were not distributed evenly across environmental gradients, with logistic regression identifying hybrids as being associated with temperature. Removal of hybrids resulted in increases in genetic differentiation (F ST ), expected heterozygosity, observed heterozygosity and the inbreeding coefficient, and different patterns of isolation by distance. After removal of hybrids and introgressed populations, mountain ash showed very little population genetic structure, with a small effect of isolation by distance, and very low global F ST (0.03). Our study shows that, in plants, decisions around provenancing of individuals for restoration depend on knowledge of whether hybridization is influencing population genetic structure. For species in which most genetic variation is held within populations, there may be little benefit in planning conservation strategies around environmental adaptation of seed sources. The possibility for adaptive introgression may also be relevant when species regularly hybridize.

    Original languageEnglish
    Pages (from-to)845-860
    Number of pages16
    JournalEvolutionary Applications
    Volume12
    Issue number4
    Early online date30 Dec 2018
    DOIs
    Publication statusPublished - Apr 2019

    Fingerprint

    Population Genetics
    Genetic Structures
    population genetics
    Eucalyptus
    genetic variation
    hybridization
    genetic structure
    Population
    heterozygosity
    Sorbus
    Eucalyptus obliqua
    Eucalyptus regnans
    Reproductive Isolation
    South Australia
    Inbreeding
    Gene Flow
    ecological restoration
    inbreeding coefficient
    reproductive isolation
    montane forests

    Cite this

    von Takach Dukai, Brenton ; Jack, Cameron ; Borevitz, Justin ; Lindenmayer, David B. ; Banks, Sam C. / Pervasive admixture between eucalypt species has consequences for conservation and assisted migration. In: Evolutionary Applications. 2019 ; Vol. 12, No. 4. pp. 845-860.
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    abstract = "Conservation management often uses information on genetic population structure to assess the importance of local provenancing for ecological restoration and reintroduction programs. For species that do not exhibit complete reproductive isolation, the estimation of population genetic parameters may be influenced by the extent of admixture. Therefore, to avoid perverse outcomes for conservation, genetically informed management strategies must determine whether hybridization between species is relevant, and the extent to which observed population genetic patterns are shaped by interspecific versus intraspecific gene flow. We used genotyping by sequencing to identify over 2,400 informative single nucleotide polymorphisms across 18 populations of Eucalyptus regnans F. Muell., a foundation tree species of montane forests in south-eastern Australia. We used these data to determine the extent of hybridization with another species, Eucalyptus obliqua L'H{\'e}r., and investigate how admixture influences genetic diversity parameters, by estimating metrics of genetic diversity and examining population genetic structure in datasets with and without admixed individuals. We found hybrid individuals at all sites and two highly introgressed populations. Hybrid individuals were not distributed evenly across environmental gradients, with logistic regression identifying hybrids as being associated with temperature. Removal of hybrids resulted in increases in genetic differentiation (F ST ), expected heterozygosity, observed heterozygosity and the inbreeding coefficient, and different patterns of isolation by distance. After removal of hybrids and introgressed populations, mountain ash showed very little population genetic structure, with a small effect of isolation by distance, and very low global F ST (0.03). Our study shows that, in plants, decisions around provenancing of individuals for restoration depend on knowledge of whether hybridization is influencing population genetic structure. For species in which most genetic variation is held within populations, there may be little benefit in planning conservation strategies around environmental adaptation of seed sources. The possibility for adaptive introgression may also be relevant when species regularly hybridize.",
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    Pervasive admixture between eucalypt species has consequences for conservation and assisted migration. / von Takach Dukai, Brenton; Jack, Cameron; Borevitz, Justin; Lindenmayer, David B.; Banks, Sam C.

    In: Evolutionary Applications, Vol. 12, No. 4, 04.2019, p. 845-860.

    Research output: Contribution to journalArticleResearchpeer-review

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