Potential use of multiplex PCR in diagnosis of tuberculous meningitis

N. Philip, C. Wilkes, T. W. Yeo, G. S. Rajahram, T. William, D. V. John

Research output: Contribution to journalArticlepeer-review


Accurate and rapid diagnosis of tuberculous meningitis (TBM) is important for early administration of treatment. In this study, we have evaluated the diagnostic value of smear, culture, multiplex PCR and GeneXpert MTB/RIF to detect M. tuberculosis in cerebrospinal fluid (CSF) samples from patients with suspected TBM registered in Queen Elizabeth Hospital, Kota Kinabalu, Sabah, Malaysia. Of the 55 CSF samples collected, 12 (21.8%) samples were positive by multiplex PCR, 3 (5.4%) by GeneXpert MTB/RIF and only 1 (1.8%) by smear and culture. Multiplex PCR showed higher sensitivity to detect M. tuberculosis in patients with suspected TBM and has the potential to be used as a diagnostic method.

Original languageEnglish
Pages (from-to)870-876
Number of pages7
JournalTropical Biomedicine
Issue number4
Publication statusPublished - 2017
Externally publishedYes


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