Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China

Ying Liang Duan, Glenn Bellis, Le Li, Hua Chun Li, Hai Sheng Miao, Mei Ling Kou, De Fang Liao, Zheng Wang, Lin Gao, Ji Zhong Li

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    BACKGROUND: Bluetongue disease of ruminants is a typical insect-borne disease caused by bluetongue virus (BTV) of the genus Orbivirus (family Reoviridae) and transmitted by some species of Culicoides (Diptera: Ceratopogonidae). Recently, the detection of BTV in yaks in high altitude meadows of the Shangri-La district of Yunnan Province, China, prompted an investigation of the Culicoides fauna as potential vectors of BTV.

    METHODS: A total of 806 Culicoides midges were collected by light trapping at three sites at altitudes ranging from 1800 to 3300 m. The species were identified based on morphology and the DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in Culicoides spp. A phylogenetic analysis was used to analyze the cox1 sequences of some specimens.

    RESULTS: Four species dominated these collections and cox1 barcoding revealed that at least two of these appear to belong to species new to science. Culicoides tainanus and a cryptic species morphologically similar to C. tainanus dominated low altitude valley collections while C. nielamensis was the most abundant species in the high-altitude meadow. A species related to C. obsoletus occurred at all altitudes but did not dominate any of the collections. BTV RT-qPCR analysis detected BTV RNA in two specimens of C. tainanus, in one specimen closely related to C. tainanus and in one specimen closely related to C. obsoletus by barcode sequencing.

    CONCLUSIONS: This study suggests that BTV in high altitude areas of Yunnan is being transmitted by three species of Culicoides, two of which appear to be new to science. This research may be useful in improving understanding of the effects of global warming on arboviral disease epidemiology and further study is important in research into disease control and prevention.

    Original languageEnglish
    Article number464
    Pages (from-to)1-11
    Number of pages11
    JournalParasites & vectors
    Volume12
    Issue number1
    DOIs
    Publication statusPublished - 4 Oct 2019

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    Ceratopogonidae
    Bluetongue virus
    China
    Oxidoreductases
    Orbivirus
    Reoviridae
    Bluetongue
    RNA
    Global Warming
    Polymerase Chain Reaction
    Ruminants
    Electron Transport Complex IV
    Research
    Diptera
    Reverse Transcription
    Insects
    Epidemiology
    Light

    Cite this

    Duan, Y. L., Bellis, G., Li, L., Li, H. C., Miao, H. S., Kou, M. L., ... Li, J. Z. (2019). Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China. Parasites & vectors, 12(1), 1-11. [464]. https://doi.org/10.1186/s13071-019-3736-9
    Duan, Ying Liang ; Bellis, Glenn ; Li, Le ; Li, Hua Chun ; Miao, Hai Sheng ; Kou, Mei Ling ; Liao, De Fang ; Wang, Zheng ; Gao, Lin ; Li, Ji Zhong. / Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China. In: Parasites & vectors. 2019 ; Vol. 12, No. 1. pp. 1-11.
    @article{e21ce209de984427aad060a1e573fd00,
    title = "Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China",
    abstract = "BACKGROUND: Bluetongue disease of ruminants is a typical insect-borne disease caused by bluetongue virus (BTV) of the genus Orbivirus (family Reoviridae) and transmitted by some species of Culicoides (Diptera: Ceratopogonidae). Recently, the detection of BTV in yaks in high altitude meadows of the Shangri-La district of Yunnan Province, China, prompted an investigation of the Culicoides fauna as potential vectors of BTV. METHODS: A total of 806 Culicoides midges were collected by light trapping at three sites at altitudes ranging from 1800 to 3300 m. The species were identified based on morphology and the DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in Culicoides spp. A phylogenetic analysis was used to analyze the cox1 sequences of some specimens. RESULTS: Four species dominated these collections and cox1 barcoding revealed that at least two of these appear to belong to species new to science. Culicoides tainanus and a cryptic species morphologically similar to C. tainanus dominated low altitude valley collections while C. nielamensis was the most abundant species in the high-altitude meadow. A species related to C. obsoletus occurred at all altitudes but did not dominate any of the collections. BTV RT-qPCR analysis detected BTV RNA in two specimens of C. tainanus, in one specimen closely related to C. tainanus and in one specimen closely related to C. obsoletus by barcode sequencing. CONCLUSIONS: This study suggests that BTV in high altitude areas of Yunnan is being transmitted by three species of Culicoides, two of which appear to be new to science. This research may be useful in improving understanding of the effects of global warming on arboviral disease epidemiology and further study is important in research into disease control and prevention.",
    keywords = "Bluetongue virus vector, C. nielamensis, C. obsoletus, C. tainanus, Culicoides, High altitude",
    author = "Duan, {Ying Liang} and Glenn Bellis and Le Li and Li, {Hua Chun} and Miao, {Hai Sheng} and Kou, {Mei Ling} and Liao, {De Fang} and Zheng Wang and Lin Gao and Li, {Ji Zhong}",
    year = "2019",
    month = "10",
    day = "4",
    doi = "10.1186/s13071-019-3736-9",
    language = "English",
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    Duan, YL, Bellis, G, Li, L, Li, HC, Miao, HS, Kou, ML, Liao, DF, Wang, Z, Gao, L & Li, JZ 2019, 'Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China', Parasites & vectors, vol. 12, no. 1, 464, pp. 1-11. https://doi.org/10.1186/s13071-019-3736-9

    Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China. / Duan, Ying Liang; Bellis, Glenn; Li, Le; Li, Hua Chun; Miao, Hai Sheng; Kou, Mei Ling; Liao, De Fang; Wang, Zheng; Gao, Lin; Li, Ji Zhong.

    In: Parasites & vectors, Vol. 12, No. 1, 464, 04.10.2019, p. 1-11.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Potential vectors of bluetongue virus in high altitude areas of Yunnan Province, China

    AU - Duan, Ying Liang

    AU - Bellis, Glenn

    AU - Li, Le

    AU - Li, Hua Chun

    AU - Miao, Hai Sheng

    AU - Kou, Mei Ling

    AU - Liao, De Fang

    AU - Wang, Zheng

    AU - Gao, Lin

    AU - Li, Ji Zhong

    PY - 2019/10/4

    Y1 - 2019/10/4

    N2 - BACKGROUND: Bluetongue disease of ruminants is a typical insect-borne disease caused by bluetongue virus (BTV) of the genus Orbivirus (family Reoviridae) and transmitted by some species of Culicoides (Diptera: Ceratopogonidae). Recently, the detection of BTV in yaks in high altitude meadows of the Shangri-La district of Yunnan Province, China, prompted an investigation of the Culicoides fauna as potential vectors of BTV. METHODS: A total of 806 Culicoides midges were collected by light trapping at three sites at altitudes ranging from 1800 to 3300 m. The species were identified based on morphology and the DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in Culicoides spp. A phylogenetic analysis was used to analyze the cox1 sequences of some specimens. RESULTS: Four species dominated these collections and cox1 barcoding revealed that at least two of these appear to belong to species new to science. Culicoides tainanus and a cryptic species morphologically similar to C. tainanus dominated low altitude valley collections while C. nielamensis was the most abundant species in the high-altitude meadow. A species related to C. obsoletus occurred at all altitudes but did not dominate any of the collections. BTV RT-qPCR analysis detected BTV RNA in two specimens of C. tainanus, in one specimen closely related to C. tainanus and in one specimen closely related to C. obsoletus by barcode sequencing. CONCLUSIONS: This study suggests that BTV in high altitude areas of Yunnan is being transmitted by three species of Culicoides, two of which appear to be new to science. This research may be useful in improving understanding of the effects of global warming on arboviral disease epidemiology and further study is important in research into disease control and prevention.

    AB - BACKGROUND: Bluetongue disease of ruminants is a typical insect-borne disease caused by bluetongue virus (BTV) of the genus Orbivirus (family Reoviridae) and transmitted by some species of Culicoides (Diptera: Ceratopogonidae). Recently, the detection of BTV in yaks in high altitude meadows of the Shangri-La district of Yunnan Province, China, prompted an investigation of the Culicoides fauna as potential vectors of BTV. METHODS: A total of 806 Culicoides midges were collected by light trapping at three sites at altitudes ranging from 1800 to 3300 m. The species were identified based on morphology and the DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in Culicoides spp. A phylogenetic analysis was used to analyze the cox1 sequences of some specimens. RESULTS: Four species dominated these collections and cox1 barcoding revealed that at least two of these appear to belong to species new to science. Culicoides tainanus and a cryptic species morphologically similar to C. tainanus dominated low altitude valley collections while C. nielamensis was the most abundant species in the high-altitude meadow. A species related to C. obsoletus occurred at all altitudes but did not dominate any of the collections. BTV RT-qPCR analysis detected BTV RNA in two specimens of C. tainanus, in one specimen closely related to C. tainanus and in one specimen closely related to C. obsoletus by barcode sequencing. CONCLUSIONS: This study suggests that BTV in high altitude areas of Yunnan is being transmitted by three species of Culicoides, two of which appear to be new to science. This research may be useful in improving understanding of the effects of global warming on arboviral disease epidemiology and further study is important in research into disease control and prevention.

    KW - Bluetongue virus vector

    KW - C. nielamensis

    KW - C. obsoletus

    KW - C. tainanus

    KW - Culicoides

    KW - High altitude

    UR - http://www.scopus.com/inward/record.url?scp=85072909306&partnerID=8YFLogxK

    U2 - 10.1186/s13071-019-3736-9

    DO - 10.1186/s13071-019-3736-9

    M3 - Article

    VL - 12

    SP - 1

    EP - 11

    JO - Parasites and Vectors

    JF - Parasites and Vectors

    SN - 1756-3305

    IS - 1

    M1 - 464

    ER -