Preserved dendritic cell HLA-DR expression and reduced regulatory T cell activation in Asymptomatic Plasmodium falciparum and P. vivax infection

Steven Kho, Jutta Marfurt, Rintis Noviyanti, Andreas Kusuma, Kim Piera, Faustina H. Burdam, Enny Kenangalem, Daniel Lampah, Christian Engwerda, Jeanne Rini Poespoprodjo, Ric Price, Nicholas Anstey, Gabriela Minigo, Tonia Woodberry

    Research output: Contribution to journalArticle

    Abstract

    Clinical illness with Plasmodium falciparum or Plasmodium vivax compromises the function of dendritic cells (DC) and expands regulatory T (Treg) cells. Individuals with asymptomatic parasitemia have clinical immunity, restricting parasite expansion and preventing clinical disease. The role of DC and Treg cells during asymptomatic Plasmodium infection is unclear. During a cross-sectional household survey in Papua, Indonesia, we examined the number and activation of blood plasmacytoid DC (pDC), CD141+, and CD1c+ myeloid DC (mDC) subsets and Treg cells using flow cytometry in 168 afebrile children (of whom 15 had P. falciparum and 36 had P. vivax infections) and 162 afebrile adults (of whom 20 had P. falciparum and 20 had P. vivax infections), alongside samples from 16 patients hospitalized with uncomplicated malaria. Unlike DC from malaria patients, DC from children and adults with asymptomatic, microscopy-positive P. vivax or P. falciparum infection increased or retained HLA-DR expression. Treg cells in asymptomatic adults and children exhibited reduced activation, suggesting increased immune responsiveness. The pDC and mDC subsets varied according to clinical immunity (asymptomatic or symptomatic Plasmodium infection) and, in asymptomatic infection, according to host age and parasite species. In conclusion, active control of asymptomatic infection was associated with and likely contingent upon functional DC and reduced Treg cell activation.
    Original languageEnglish
    Pages (from-to)3224-3232
    Number of pages9
    JournalInfection and Immunity
    Volume83
    Issue number8
    DOIs
    Publication statusPublished - Aug 2015

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  • Cite this

    Kho, S., Marfurt, J., Noviyanti, R., Kusuma, A., Piera, K., Burdam, F. H., Kenangalem, E., Lampah, D., Engwerda, C., Poespoprodjo, J. R., Price, R., Anstey, N., Minigo, G., & Woodberry, T. (2015). Preserved dendritic cell HLA-DR expression and reduced regulatory T cell activation in Asymptomatic Plasmodium falciparum and P. vivax infection. Infection and Immunity, 83(8), 3224-3232. https://doi.org/10.1128/IAI.00226-15