Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis

Luke Norbury, Rama Jayaraj, Robert N Pike, Terry W Spithill, Peter M Smooker

Research output: Chapter in Book/Report/Conference proceedingConference Paper published in Proceedings

Abstract

Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material.Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L’s are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L’s and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalentvaccine may be optimal. 
Original languageEnglish
Title of host publicationProceedings of 2010 International Conference on Agriculture and Animal Science
EditorsSaji Baby, Parvinder Singh Sandhu, Yi Hang
Place of PublicationEngland, UK.
PublisherWorld Academic Union
Pages1-5
Number of pages5
ISBN (Print)9781846260223
Publication statusPublished - 2010
Event2010 International Conference on Agriculture and Animal Science - Singapore, Singapore
Duration: 26 Feb 201028 Feb 2010

Conference

Conference2010 International Conference on Agriculture and Animal Science
Period26/02/1028/02/10

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  • Cite this

    Norbury, L., Jayaraj, R., Pike, R. N., Spithill, T. W., & Smooker, P. M. (2010). Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. In S. Baby, P. S. Sandhu, & Y. Hang (Eds.), Proceedings of 2010 International Conference on Agriculture and Animal Science (pp. 1-5). World Academic Union.