Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis

Luke Norbury, Rama Jayaraj, Robert N Pike, Terry W Spithill, Peter M Smooker

Research output: Chapter in Book/Report/Conference proceedingConference Paper published in ProceedingsResearchpeer-review

Abstract

Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material.Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L’s are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L’s and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalentvaccine may be optimal. 
Original languageEnglish
Title of host publicationProceedings of 2010 International Conference on Agriculture and Animal Science
EditorsSaji Baby, Parvinder Singh Sandhu, Yi Hang
Place of PublicationEngland, UK.
PublisherWorld Academic Union
Pages1-5
Number of pages5
ISBN (Print)9781846260223
Publication statusPublished - 2010
Event2010 International Conference on Agriculture and Animal Science - Singapore, Singapore
Duration: 26 Feb 201028 Feb 2010

Conference

Conference2010 International Conference on Agriculture and Animal Science
Period26/02/1028/02/10

Fingerprint

cathepsin L
cathepsins
cathepsin B
liver flukes
fascioliasis
proteinases
vaccination
developmental stages
vaccines
yeasts
antigens
phylogeny
proteins

Cite this

Norbury, L., Jayaraj, R., Pike, R. N., Spithill, T. W., & Smooker, P. M. (2010). Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. In S. Baby, P. S. Sandhu, & Y. Hang (Eds.), Proceedings of 2010 International Conference on Agriculture and Animal Science (pp. 1-5). England, UK.: World Academic Union.
Norbury, Luke ; Jayaraj, Rama ; Pike, Robert N ; Spithill, Terry W ; Smooker, Peter M. / Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. Proceedings of 2010 International Conference on Agriculture and Animal Science. editor / Saji Baby ; Parvinder Singh Sandhu ; Yi Hang. England, UK. : World Academic Union, 2010. pp. 1-5
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abstract = "Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material.Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L’s are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L’s and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalentvaccine may be optimal. ",
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Norbury, L, Jayaraj, R, Pike, RN, Spithill, TW & Smooker, PM 2010, Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. in S Baby, PS Sandhu & Y Hang (eds), Proceedings of 2010 International Conference on Agriculture and Animal Science. World Academic Union, England, UK., pp. 1-5, 2010 International Conference on Agriculture and Animal Science, 26/02/10.

Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. / Norbury, Luke; Jayaraj, Rama; Pike, Robert N; Spithill, Terry W; Smooker, Peter M.

Proceedings of 2010 International Conference on Agriculture and Animal Science. ed. / Saji Baby; Parvinder Singh Sandhu; Yi Hang. England, UK. : World Academic Union, 2010. p. 1-5.

Research output: Chapter in Book/Report/Conference proceedingConference Paper published in ProceedingsResearchpeer-review

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T1 - Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis

AU - Norbury, Luke

AU - Jayaraj, Rama

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AU - Spithill, Terry W

AU - Smooker, Peter M

PY - 2010

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N2 - Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material.Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L’s are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L’s and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalentvaccine may be optimal. 

AB - Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material.Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L’s are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L’s and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalentvaccine may be optimal. 

M3 - Conference Paper published in Proceedings

SN - 9781846260223

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Norbury L, Jayaraj R, Pike RN, Spithill TW, Smooker PM. Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. In Baby S, Sandhu PS, Hang Y, editors, Proceedings of 2010 International Conference on Agriculture and Animal Science. England, UK.: World Academic Union. 2010. p. 1-5