Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus

Kate Mounsey, C Willis, Stewart Burgess, Deborah Holt, James McCarthy, Katja Fischer

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    Abstract

    Background:  The lack of genomic data available for mites limits our understanding of their biology. Evolving highthroughput sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome size are initially required to ensure sufficient coverage.

    Methods:
      Quantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite Sarcoptes scabiei, the non-burrowing ectoparasitic mite Psoroptes ovis, and the free-living house dust mite Dermatophagoides pteronyssinus. Additionally, the chromosome number of S. scabiei was determined by chromosomal spreads of embryonic cells derived from single eggs.

    Results: 
    S. scabiei cells were shown to contain 17 or 18 small (< 2 μM) chromosomes, suggesting an XO sexdetermination mechanism. The average estimated genome sizes of S. scabiei and P. ovis were 96 (± 7) Mb and 86 (± 2) Mb respectively, among the smallest arthropod genomes reported to date. The D. pteronyssinus genome was estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites.

    Conclusions: 
    This data provides a starting point for understanding the genetic organisation and evolution of theseastigmatid mites, informing future sequencing projects. A comparitive genomic approach including these threeclosely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.
    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalParasites and Vectors
    Volume5
    Issue number3
    DOIs
    Publication statusPublished - 2012

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