@article{1c0c9d3ba76a4bc0a6fa434e03f1631c,
title = "Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis",
abstract = "Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection. ",
keywords = "article, bacterial count, bacterium culture, bacterium detection, bacterium identification, bronchiectasis, child, controlled study, diagnostic test accuracy study, female, Haemophilus infection, Haemophilus influenzae, human, indigenous people, infant, intermethod comparison, lower respiratory tract infection, lung lavage, major clinical study, male, neutrophilia, nonhuman, polymerase chain reaction, priority journal, quantitative analysis, school child, sensitivity and specificity, Australia, Bacteriological Techniques, Bronchiectasis, Bronchoalveolar Lavage Fluid, Bronchopneumonia, Child, Child, Preschool, Female, Haemophilus Infections, Humans, Infant, Male, Molecular Diagnostic Techniques, Population Groups, Real-Time Polymerase Chain Reaction",
author = "Kim Hare and Robyn Marsh and Michael Binks and Keith Grimwood and Susan Pizzutto and Amanda Leach and Anne Chang and Heidi Smith-Vaughan",
year = "2013",
month = mar,
doi = "10.1016/j.mimet.2012.12.013",
language = "English",
volume = "92",
pages = "270--272",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "3",
}