Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

Kim Hare, Robyn Marsh, Michael Binks, Keith Grimwood, Susan Pizzutto, Amanda Leach, Anne Chang, Heidi Smith-Vaughan

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    Abstract

    Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
    Original languageEnglish
    Pages (from-to)270-272
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume92
    Issue number3
    DOIs
    Publication statusPublished - Mar 2013

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