TY - JOUR
T1 - Rapid design and fielding of four diagnostic technologies in Sierra Leone, Thailand, Peru, and Australia
T2 - Successes and challenges faced introducing these biosensors
AU - Mulvaney, Shawn P.
AU - Fitzgerald, Lisa A.
AU - Hamdan, Leila J.
AU - Ringeisen, Bradley R.
AU - Petersen, Emily R.
AU - Compton, Jaimee R.
AU - McAuliff, Nina L.
AU - Leski, Tomasz A.
AU - Taitt, Chris R.
AU - Stenger, David A.
AU - Myers, Christopher A.
AU - Hansen, Erin
AU - Ricketts, Michelle
AU - Hoegberg, Chelsea
AU - Homdayjanakul, Kamonthip
AU - Ansumana, Rashid
AU - Lamin, Joseph M.
AU - Bangura, Umaru
AU - Lahai, Joseph
AU - Baio, Victoria
AU - Limmathurotsakul, Direk
AU - Wongsuvan, Gumphol
AU - Hantrakun, Viriya
AU - Wacharapluesadee, Supaporn
AU - Mungaomklang, Anek
AU - Putcharoen, Opass
AU - Yatoom, Pratoomtong
AU - Kruthakool, Kriengsak
AU - Hontz, Robert D.
AU - Mores, Christopher
AU - Siles, Crystyan
AU - Morrison, Amy
AU - Mayo, Mark
AU - Currie, Bart J.
AU - Jacobsen, Kathryn H.
AU - Quinn, Kathleen
AU - Blutman, Jerold
AU - Amariei, Flavia
AU - Hannan, John
PY - 2018/9
Y1 - 2018/9
N2 - Febrile illnesses are among the most common reasons for visits to hospitals and clinics worldwide. Since fevers can arise from a wide range of diseases, identifying the causative pathogen is essential not only for effective personal treatment but also for early detection of outbreaks. The Defense Threat Reduction Agency (DTRA) tasked a coalition of commercial, academic, and government researchers with moving diagnostic technology concepts from ideation to field use as rapidly as possible using scientifically sound evaluations. DTRA's 24 Month Challenge program examined >30 technologies before fielding four technologies on four continents. >10,000 in field test results were recorded. Here we discuss our tiered evaluation system to assess candidate technologies developed by commercial partners and the process of field testing those technologies at various front-line clinics in Sierra Leone, Thailand, Peru, and Australia. We discuss successes and challenges for introducing two multiplexed lateral flow immunoassay (LFI) tests that detect malaria, dengue fever, melioidosis, and the plague. Additionally we discuss the use of a LFI reader that assisted the interpretation of the assay, communicated results to a data cloud, and greatly facilitated reach-back support. Lastly, we discuss the concurrent field testing of a multiplexed PCR assay on the FilmArray platform, which had an assay pouch specially designed for the 24 Month Challenge. Either standard-of-care or gold-standard testing were run alongside our fielded technologies to benchmark their performance.
AB - Febrile illnesses are among the most common reasons for visits to hospitals and clinics worldwide. Since fevers can arise from a wide range of diseases, identifying the causative pathogen is essential not only for effective personal treatment but also for early detection of outbreaks. The Defense Threat Reduction Agency (DTRA) tasked a coalition of commercial, academic, and government researchers with moving diagnostic technology concepts from ideation to field use as rapidly as possible using scientifically sound evaluations. DTRA's 24 Month Challenge program examined >30 technologies before fielding four technologies on four continents. >10,000 in field test results were recorded. Here we discuss our tiered evaluation system to assess candidate technologies developed by commercial partners and the process of field testing those technologies at various front-line clinics in Sierra Leone, Thailand, Peru, and Australia. We discuss successes and challenges for introducing two multiplexed lateral flow immunoassay (LFI) tests that detect malaria, dengue fever, melioidosis, and the plague. Additionally we discuss the use of a LFI reader that assisted the interpretation of the assay, communicated results to a data cloud, and greatly facilitated reach-back support. Lastly, we discuss the concurrent field testing of a multiplexed PCR assay on the FilmArray platform, which had an assay pouch specially designed for the 24 Month Challenge. Either standard-of-care or gold-standard testing were run alongside our fielded technologies to benchmark their performance.
KW - Dengue fever
KW - Diagnostic device
KW - Field testing
KW - Lateral flow immunoassay
KW - Malaria
KW - Nested PCR
UR - http://www.scopus.com/inward/record.url?scp=85049308452&partnerID=8YFLogxK
U2 - 10.1016/j.sbsr.2018.06.003
DO - 10.1016/j.sbsr.2018.06.003
M3 - Article
AN - SCOPUS:85049308452
SN - 2214-1804
VL - 20
SP - 22
EP - 33
JO - Sensing and Bio-Sensing Research
JF - Sensing and Bio-Sensing Research
ER -