Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Steven Tong, Rachael Gorie, Deborah Holt, Geoffrey Coombs, Bart Currie, Philip Giffard

    Research output: Contribution to journalArticle

    Abstract

    We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.
    Original languageEnglish
    Pages (from-to)399-401
    Number of pages3
    JournalDiagnostic Microbiology and Infectious Disease
    Volume67
    Publication statusPublished - 2010

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