@article{0ebcd0e951ea4f2392743cf6e3c328d1,
title = "Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis",
abstract = "We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.",
keywords = "arginine, histidine, meticillin, Panton Valentine leukocidin, bacterial protein, bacterial toxin, exotoxin, isoprotein, leukocidin, Panton-Valentine leukocidin, article, Australia, bacterium detection, bacterium isolate, DNA sequence, high resolution melting analysis, image analysis, nonhuman, priority journal, real time polymerase chain reaction, sensitivity and specificity, single nucleotide polymorphism, Staphylococcus aureus, genetics, human, isolation and purification, microbiology, polymerase chain reaction, procedures, Staphylococcus infection, transition temperature, Bacterial Proteins, Bacterial Toxins, Exotoxins, Humans, Leukocidins, Polymerase Chain Reaction, Protein Isoforms, Staphylococcal Infections, Transition Temperature",
author = "Steven Tong and Rachael Gorie and Deborah Holt and Geoffrey Coombs and Bart Currie and Philip Giffard",
year = "2010",
language = "English",
volume = "67",
pages = "399--401",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier",
}