Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Steven Tong; Rachael Gorie; Deborah Holt; Geoffrey Coombs; Bart Currie; Philip Giffard

Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Steven Tong, Rachael Gorie, Deborah Holt, Geoffrey Coombs, Bart Currie, Philip Giffard

Global and Tropical Health

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.

Original language	English
Pages (from-to)	399-401
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	67
Publication status	Published - 2010

Cite this

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title = "Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis",

abstract = "We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.",

keywords = "arginine, histidine, meticillin, Panton Valentine leukocidin, bacterial protein, bacterial toxin, exotoxin, isoprotein, leukocidin, Panton-Valentine leukocidin, article, Australia, bacterium detection, bacterium isolate, DNA sequence, high resolution melting analysis, image analysis, nonhuman, priority journal, real time polymerase chain reaction, sensitivity and specificity, single nucleotide polymorphism, Staphylococcus aureus, genetics, human, isolation and purification, microbiology, polymerase chain reaction, procedures, Staphylococcus infection, transition temperature, Bacterial Proteins, Bacterial Toxins, Exotoxins, Humans, Leukocidins, Polymerase Chain Reaction, Protein Isoforms, Staphylococcal Infections, Transition Temperature",

author = "Steven Tong and Rachael Gorie and Deborah Holt and Geoffrey Coombs and Bart Currie and Philip Giffard",

year = "2010",

language = "English",

volume = "67",

pages = "399--401",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

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TY - JOUR

T1 - Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

AU - Tong, Steven

AU - Gorie, Rachael

AU - Holt, Deborah

AU - Coombs, Geoffrey

AU - Currie, Bart

AU - Giffard, Philip

PY - 2010

Y1 - 2010

N2 - We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.

AB - We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. � 2010.

KW - arginine

KW - histidine

KW - meticillin

KW - Panton Valentine leukocidin

KW - bacterial protein

KW - bacterial toxin

KW - exotoxin

KW - isoprotein

KW - leukocidin

KW - Panton-Valentine leukocidin

KW - article

KW - Australia

KW - bacterium detection

KW - bacterium isolate

KW - DNA sequence

KW - high resolution melting analysis

KW - image analysis

KW - nonhuman

KW - priority journal

KW - real time polymerase chain reaction

KW - sensitivity and specificity

KW - single nucleotide polymorphism

KW - Staphylococcus aureus

KW - genetics

KW - human

KW - isolation and purification

KW - microbiology

KW - polymerase chain reaction

KW - procedures

KW - Staphylococcus infection

KW - transition temperature

KW - Bacterial Proteins

KW - Bacterial Toxins

KW - Exotoxins

KW - Humans

KW - Leukocidins

KW - Polymerase Chain Reaction

KW - Protein Isoforms

KW - Staphylococcal Infections

KW - Transition Temperature

UR - https://www.scopus.com/pages/publications/77954735663

M3 - Article

SN - 0732-8893

VL - 67

SP - 399

EP - 401

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

ER -

Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Abstract

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