Rapid immunofluorescence microscopy for diagnosis of melioidosis

Vanaporn Wuthiekanun, Varunee Desakorn, Gumphol Wongsuvan, Premjit Amornchai, Allen Cheng, Bina Maharjan, Direk Limmathursakul, Wirongrong Chierakul, Nicholas J. White, Nicholas P. J. Day, Sharon J. Peacock

Research output: Contribution to journalComment/debate


An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared withthe culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.
Original languageEnglish
Pages (from-to)555-556
Number of pages2
JournalClinical and Vaccine Immunology
Issue number4
Publication statusPublished - 2005


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