Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: A retrospective re-analysis

Jemima Beissbarth; Michael J. Binks; Robyn L. Marsh; Anne B. Chang; Amanda J. Leach; Heidi C. Smith-Vaughan

doi:10.1186/s13104-018-3429-z

Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: A retrospective re-analysis

Jemima Beissbarth, Michael J. Binks, Robyn L. Marsh, Anne B. Chang, Amanda J. Leach, Heidi C. Smith-Vaughan

Child and Maternal Health

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Abstract

Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia.

Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.

Original language	English
Article number	323
Pages (from-to)	1-5
Number of pages	5
Journal	BMC Research Notes
Volume	11
DOIs	https://doi.org/10.1186/s13104-018-3429-z
Publication status	Published - 21 May 2018

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20938815-oaFinal published version, 995 KBLicence: CC BY

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title = "Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: A retrospective re-analysis",

abstract = "Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.",

keywords = "Haemophilus haemolyticus (Hh), Nasopharynx, bronchoalveolar lavage, Nontypeable Haemophilus influenzae (NTHi)",

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AU - Beissbarth, Jemima

AU - Binks, Michael J.

AU - Marsh, Robyn L.

AU - Chang, Anne B.

AU - Leach, Amanda J.

AU - Smith-Vaughan, Heidi C.

PY - 2018/5/21

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N2 - Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.

AB - Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.

KW - Haemophilus haemolyticus (Hh)

KW - Nasopharynx, bronchoalveolar lavage

KW - Nontypeable Haemophilus influenzae (NTHi)

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Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: A retrospective re-analysis

Abstract

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