Recovery of a Burkholderia thailandensis-like isolate from an Australian water source

Background. Burkholderia thailandensis, a close relative of Burkholderia pseudomallei, has previously been reported only from Southeast Asia and North America. It is biochemically differentiated from B. pseudomallei by the ability to utilize arabinose. During the course of environmental sampling for B. pseudomallei in the Northern Territory of Australia, an isolate, MSMB 43, was recovered that is arabinose positive. Results. Genetic analysis using 16S rDNA sequencing and DNA/DNA hybridization indicates that MSMB 43 is most similar to B. thailandensis although multi-locus sequence typing indicates that this isolate is divergent from both B. pseudomallei and other described B. thailandensis. Conclusion. We report the isolation and initial characterization of strain MSMB 43, which is a B. thailandensis-like isolate recovered in Australia. � 2008 Gee et al; licensee BioMed Central Ltd.