Results from an inter-laboratory comparison of pneumococcal serotype-specific IgG measurement and critical parameters that affect assay performance

Anne Balloch, Paul Licciardi, Amanda Leach, A Nurkka, Mimi Tang

    Research output: Contribution to journalArticle

    Abstract

    Quantitation of specific IgG to polysaccharides (serotypes) of Streptococcus pneumoniae provides the basis for evaluating vaccine efficacy. Different enzyme-linked immunosorbent assay (ELISA) methods are used internationally, making comparisons between laboratories difficult. We undertook an inter-laboratory comparison between two international laboratories performing serotype-specific IgG ELISAs using a panel of well-characterized serum samples: the Murdoch Childrens Research Institute Pneumococcal Laboratory (Melbourne, Australia) and the Vaccine Immunology Laboratory, National Public Health Institute (Helsinki, Finland). While good agreement was found for the inter-laboratory comparison for most serotypes, differences in ELISA methodology influenced specific IgG measurement. Therefore, use of the World Health Organization (WHO)-based ELISA methods for measurement of serotype-specific IgG is reliable, accurate and provides consistent results between international laboratories. � 2009 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)1333-1340
    Number of pages8
    JournalVaccine
    Volume28
    Issue number5
    DOIs
    Publication statusPublished - Feb 2010

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