TY - JOUR
T1 - Retrospective analysis of Cryptosporidium species in Western Australian human populations (2015–2018), and emergence of the C. hominis IfA12G1R5 subtype
AU - Braima, Kamil
AU - Zahedi, Alireza
AU - Oskam, Charlotte
AU - Reid, Simon
AU - Pingault, Nevada
AU - Xiao, Lihua
AU - Ryan, Una
N1 - Funding Information:
The authors wish to acknowledge the assistance of Frances Brigg and the Western Australia State Agriculture Biotechnology Centre (SABC) for Sanger sequencing, and staff from the Enteric Laboratory of PathWest Laboratory Medicine WA for their assistance in obtaining the faecal samples. This work was supported by Australian Research Council Linkage Grant (Grant number LP130100035 ).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/9
Y1 - 2019/9
N2 - Cryptosporidium species are a major cause of diarrhoea worldwide. In the present study, a retrospective analysis of 109 microscopically Cryptosporidium-positive faecal specimens from Western Australian patients, collected between 2015 and 2018 was conducted. Sequence analysis of the 18S rRNA and the 60 kDa glycoprotein (gp60)gene loci identified four Cryptosporidium species: C. hominis (86.2%, 94/109), C. parvum (11.0%, 12/109), C. meleagridis (1.8%, 2/109) and C. viatorum (0.9%, 1/109). Subtyping at the gp60 locus identified a total of 11 subtypes including the emergence of the previously rare C. hominis IfA12G1R5 subtype in 2017 as the dominant subtype (46.7%, 21/45). This subtype has also recently emerged as the dominant subtype in the United States but the reasons for its emergence are unknown. This is also the first report of C. viatorum in humans in Australia and a novel subtype (XVaA3g) was identified in the one positive patient.
AB - Cryptosporidium species are a major cause of diarrhoea worldwide. In the present study, a retrospective analysis of 109 microscopically Cryptosporidium-positive faecal specimens from Western Australian patients, collected between 2015 and 2018 was conducted. Sequence analysis of the 18S rRNA and the 60 kDa glycoprotein (gp60)gene loci identified four Cryptosporidium species: C. hominis (86.2%, 94/109), C. parvum (11.0%, 12/109), C. meleagridis (1.8%, 2/109) and C. viatorum (0.9%, 1/109). Subtyping at the gp60 locus identified a total of 11 subtypes including the emergence of the previously rare C. hominis IfA12G1R5 subtype in 2017 as the dominant subtype (46.7%, 21/45). This subtype has also recently emerged as the dominant subtype in the United States but the reasons for its emergence are unknown. This is also the first report of C. viatorum in humans in Australia and a novel subtype (XVaA3g) was identified in the one positive patient.
KW - 18S rRNA
KW - C. viatorum
KW - Cryptosporidium hominis
KW - gp60
KW - Subtype IfA12G1R5
UR - http://www.scopus.com/inward/record.url?scp=85066236408&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2019.05.018
DO - 10.1016/j.meegid.2019.05.018
M3 - Article
C2 - 31146044
AN - SCOPUS:85066236408
VL - 73
SP - 306
EP - 313
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
ER -