Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 108-115 |
| Number of pages | 8 |
| Journal | Developmental and Comparative Immunology |
| Volume | 51 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2015 |
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Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus). / Milic, Natalie; Davis, Stephen; Carr, Jillian; Isberg, Sally; Beard, Michael; Helbig, Karla.
In: Developmental and Comparative Immunology, Vol. 51, No. 1, 07.2015, p. 108-115.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus)
AU - Milic, Natalie
AU - Davis, Stephen
AU - Carr, Jillian
AU - Isberg, Sally
AU - Beard, Michael
AU - Helbig, Karla
PY - 2015/7
Y1 - 2015/7
N2 - A number of pathogens have been detected in crocodiles, however little is known about their ability to control these pathogens. The interferon stimulated gene (ISG), viperin, has gained attention recently as an important host protein involved in multiple arms of the immune response. Viperin in concert with a number of other ISGs was upregulated in response to viral nucleic acid mimics and sendai virus in the C. porosus cell line, LV-1, indicating an intact early innate response to viral infection in these animals for the first time. Viperin was cloned from the LV-1 cell line and shown to have similar localisation patterns as human viperin, as well as demonstrating extremely high conservation with the human orthologue, excepting at the N-terminus. Interestingly, C. porosus viperin was also able to inhibit Dengue virus replication in vitro, showing a high level of intact functionality for this protein across divergent animal species, and perhaps demonstrating its importance in the early innate response to pathogens in the animal kingdom.
AB - A number of pathogens have been detected in crocodiles, however little is known about their ability to control these pathogens. The interferon stimulated gene (ISG), viperin, has gained attention recently as an important host protein involved in multiple arms of the immune response. Viperin in concert with a number of other ISGs was upregulated in response to viral nucleic acid mimics and sendai virus in the C. porosus cell line, LV-1, indicating an intact early innate response to viral infection in these animals for the first time. Viperin was cloned from the LV-1 cell line and shown to have similar localisation patterns as human viperin, as well as demonstrating extremely high conservation with the human orthologue, excepting at the N-terminus. Interestingly, C. porosus viperin was also able to inhibit Dengue virus replication in vitro, showing a high level of intact functionality for this protein across divergent animal species, and perhaps demonstrating its importance in the early innate response to pathogens in the animal kingdom.
KW - cell protein
KW - unclassified drug
KW - viperin protein
KW - amino terminal sequence
KW - animal cell
KW - aquatic species
KW - Article
KW - controlled study
KW - crocodile
KW - Crocodylus porosus
KW - Dengue virus
KW - gene
KW - gene frequency
KW - gene function
KW - gene location
KW - genetic analysis
KW - genetic conservation
KW - in vitro study
KW - innate immunity
KW - molecular cloning
KW - nonhuman
KW - priority journal
KW - protein expression
KW - protein localization
KW - Sendai virus
KW - sequence analysis
KW - upregulation
KW - viperin gene
KW - virus inhibition
KW - Animalia
KW - Crocodylidae (all crocodiles)
UR - http://www.scopus.com/inward/record.url?scp=84924952113&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2015.03.001
DO - 10.1016/j.dci.2015.03.001
M3 - Article
VL - 51
SP - 108
EP - 115
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
IS - 1
ER -