Sequences of multiple bacterial genomes and a Chlamydia trachomatis genotype from direct sequencing of DNA derived from a vaginal swab diagnostic specimen

Patiyan Andersson, Martin Klein, Rachael Lilliebridge, Philip Giffard

    Research output: Contribution to journalArticle

    Abstract

    Ultra-deep Illumina sequencing was performed on whole genome amplified DNA derived from a Chlamydia trachomatis-positive vaginal swab. Alignment of reads with reference genomes allowed robust SNP identification from the C. trachomatis chromosome and plasmid. This revealed that the C. trachomatis in the specimen was very closely related to the sequenced urogenital, serovar F, clade T1 isolate F-SW4. In addition, high genome-wide coverage was obtained for Prevotella melaninogenica, Gardnerella vaginalis, Clostridiales genomosp. BVAB3 and Mycoplasma hominis. This illustrates the potential of metagenome data to provide high resolution bacterial typing data from multiple taxa in a diagnostic specimen.
    Original languageEnglish
    Pages (from-to)E405-E408
    Number of pages4
    JournalClinical Microbiology and Infection
    Volume19
    Issue number9
    DOIs
    Publication statusPublished - Sep 2013

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