Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites

Philip Bejon, Louise Turner, Thomas Lavstsen, Gerald Cham, Ally Olotu, Chris J. Drakeley, Marc Lievens, Johan Vekemans, Barbara Savarese, John Lusingu, Lorenz von Seidlein, Peter C. Bull, Kevin Marsh, Thor G. Theander

    Research output: Contribution to journalArticle

    Abstract

    Background: Malaria transmission may be considered to be homogenous with well-mixed parasite populations (as in the classic Ross/Macdonald models). Marked fine-scale heterogeneity of transmission has been observed in the field (i.e., over a few kilometres), but there are relatively few data on the degree of mixing. Since the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is highly polymorphic, the host's serological responses may be used to infer exposure to parasite sub-populations.

    Methods and Findings: We measured the antibody responses to 46 individual PfEMP1 domains at four time points among 450 children in Kenya, and identified distinct spatial clusters of antibody responses to individual domains. 35 domains showed strongly significant sero-clusters at p = 0.001. Individuals within the high transmission hotspot showed the greatest diversity of anti-PfEMP1 responses. Individuals outside the hotspot had a less diverse range of responses, even if as individuals they were at relatively intense exposure.

    Conclusions: We infer that antigenically distinct sub-populations of parasites exist on a fine spatial scale in a study area of rural Kenya. Further studies should examine antigenic variation over longer periods of time and in different study areas.

    Original languageEnglish
    Article numbere21711
    JournalPLoS One
    Volume6
    Issue number6
    DOIs
    Publication statusPublished - 29 Jun 2011

    Fingerprint

    Plasmodium falciparum
    membrane proteins
    Parasites
    erythrocytes
    Kenya
    parasites
    Antibody Formation
    Population
    Antigenic Variation
    antigenic variation
    antibodies
    Antibodies
    rural areas
    malaria
    Malaria
    Plasmodium falciparum erythrocyte membrane protein 1
    methodology

    Cite this

    Bejon, P., Turner, L., Lavstsen, T., Cham, G., Olotu, A., Drakeley, C. J., ... Theander, T. G. (2011). Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites. PLoS One, 6(6), [e21711]. https://doi.org/10.1371/journal.pone.0021711
    Bejon, Philip ; Turner, Louise ; Lavstsen, Thomas ; Cham, Gerald ; Olotu, Ally ; Drakeley, Chris J. ; Lievens, Marc ; Vekemans, Johan ; Savarese, Barbara ; Lusingu, John ; von Seidlein, Lorenz ; Bull, Peter C. ; Marsh, Kevin ; Theander, Thor G. / Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites. In: PLoS One. 2011 ; Vol. 6, No. 6.
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    abstract = "Background: Malaria transmission may be considered to be homogenous with well-mixed parasite populations (as in the classic Ross/Macdonald models). Marked fine-scale heterogeneity of transmission has been observed in the field (i.e., over a few kilometres), but there are relatively few data on the degree of mixing. Since the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is highly polymorphic, the host's serological responses may be used to infer exposure to parasite sub-populations. Methods and Findings: We measured the antibody responses to 46 individual PfEMP1 domains at four time points among 450 children in Kenya, and identified distinct spatial clusters of antibody responses to individual domains. 35 domains showed strongly significant sero-clusters at p = 0.001. Individuals within the high transmission hotspot showed the greatest diversity of anti-PfEMP1 responses. Individuals outside the hotspot had a less diverse range of responses, even if as individuals they were at relatively intense exposure. Conclusions: We infer that antigenically distinct sub-populations of parasites exist on a fine spatial scale in a study area of rural Kenya. Further studies should examine antigenic variation over longer periods of time and in different study areas.",
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    author = "Philip Bejon and Louise Turner and Thomas Lavstsen and Gerald Cham and Ally Olotu and Drakeley, {Chris J.} and Marc Lievens and Johan Vekemans and Barbara Savarese and John Lusingu and {von Seidlein}, Lorenz and Bull, {Peter C.} and Kevin Marsh and Theander, {Thor G.}",
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    Bejon, P, Turner, L, Lavstsen, T, Cham, G, Olotu, A, Drakeley, CJ, Lievens, M, Vekemans, J, Savarese, B, Lusingu, J, von Seidlein, L, Bull, PC, Marsh, K & Theander, TG 2011, 'Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites', PLoS One, vol. 6, no. 6, e21711. https://doi.org/10.1371/journal.pone.0021711

    Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites. / Bejon, Philip; Turner, Louise; Lavstsen, Thomas; Cham, Gerald; Olotu, Ally; Drakeley, Chris J.; Lievens, Marc; Vekemans, Johan; Savarese, Barbara; Lusingu, John; von Seidlein, Lorenz; Bull, Peter C.; Marsh, Kevin; Theander, Thor G.

    In: PLoS One, Vol. 6, No. 6, e21711, 29.06.2011.

    Research output: Contribution to journalArticle

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    AU - Bejon, Philip

    AU - Turner, Louise

    AU - Lavstsen, Thomas

    AU - Cham, Gerald

    AU - Olotu, Ally

    AU - Drakeley, Chris J.

    AU - Lievens, Marc

    AU - Vekemans, Johan

    AU - Savarese, Barbara

    AU - Lusingu, John

    AU - von Seidlein, Lorenz

    AU - Bull, Peter C.

    AU - Marsh, Kevin

    AU - Theander, Thor G.

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    AB - Background: Malaria transmission may be considered to be homogenous with well-mixed parasite populations (as in the classic Ross/Macdonald models). Marked fine-scale heterogeneity of transmission has been observed in the field (i.e., over a few kilometres), but there are relatively few data on the degree of mixing. Since the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is highly polymorphic, the host's serological responses may be used to infer exposure to parasite sub-populations. Methods and Findings: We measured the antibody responses to 46 individual PfEMP1 domains at four time points among 450 children in Kenya, and identified distinct spatial clusters of antibody responses to individual domains. 35 domains showed strongly significant sero-clusters at p = 0.001. Individuals within the high transmission hotspot showed the greatest diversity of anti-PfEMP1 responses. Individuals outside the hotspot had a less diverse range of responses, even if as individuals they were at relatively intense exposure. Conclusions: We infer that antigenically distinct sub-populations of parasites exist on a fine spatial scale in a study area of rural Kenya. Further studies should examine antigenic variation over longer periods of time and in different study areas.

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    Bejon P, Turner L, Lavstsen T, Cham G, Olotu A, Drakeley CJ et al. Serologial evidence ofdiscrete spatial clusters of Plasmodium falciparum parasites. PLoS One. 2011 Jun 29;6(6). e21711. https://doi.org/10.1371/journal.pone.0021711