Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis

D Gal, Mark Mayo, E Spencer, A CHENG, Bart Currie

    Research output: Contribution to journalArticle

    Abstract

    The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
    Original languageEnglish
    Pages (from-to)1162-1164
    Number of pages3
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume73
    Issue number6
    Publication statusPublished - 2005

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