Short report

Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis

D Gal, Mark Mayo, E Spencer, A CHENG, Bart Currie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)1162-1164
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume73
Issue number6
Publication statusPublished - 2005

Fingerprint

Melioidosis
Burkholderia pseudomallei
Polymerase Chain Reaction
Northern Territory
Pharynx
Sputum
Nose
Rectum
Sepsis
Joints
Urine
Skin
DNA

Cite this

@article{af83bc64339c4d8d9331ec9738098468,
title = "Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis",
abstract = "The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65{\%} and the specificity was 100{\%}, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.",
keywords = "article, Australia, bacterium culture, bacterium detection, blood, Burkholderia pseudomallei, clinical article, DNA extraction, endemic disease, human, melioidosis, pericardial effusion, pleura fluid, polymerase chain reaction, sensitivity and specificity, sepsis, sputum, synovial fluid, urine, DNA, Bacterial, Humans, Melioidosis, Northern Territory, Polymerase Chain Reaction, Predictive Value of Tests, Sensitivity and Specificity, Bacteria (microorganisms)",
author = "D Gal and Mark Mayo and E Spencer and A CHENG and Bart Currie",
year = "2005",
language = "English",
volume = "73",
pages = "1162--1164",
journal = "The American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "6",

}

Short report : Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. / Gal, D; Mayo, Mark; Spencer, E; CHENG, A; Currie, Bart.

In: American Journal of Tropical Medicine and Hygiene, Vol. 73, No. 6, 2005, p. 1162-1164.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Short report

T2 - Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis

AU - Gal, D

AU - Mayo, Mark

AU - Spencer, E

AU - CHENG, A

AU - Currie, Bart

PY - 2005

Y1 - 2005

N2 - The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.

AB - The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.

KW - article

KW - Australia

KW - bacterium culture

KW - bacterium detection

KW - blood

KW - Burkholderia pseudomallei

KW - clinical article

KW - DNA extraction

KW - endemic disease

KW - human

KW - melioidosis

KW - pericardial effusion

KW - pleura fluid

KW - polymerase chain reaction

KW - sensitivity and specificity

KW - sepsis

KW - sputum

KW - synovial fluid

KW - urine

KW - DNA, Bacterial

KW - Humans

KW - Melioidosis

KW - Northern Territory

KW - Polymerase Chain Reaction

KW - Predictive Value of Tests

KW - Sensitivity and Specificity

KW - Bacteria (microorganisms)

M3 - Article

VL - 73

SP - 1162

EP - 1164

JO - The American Journal of Tropical Medicine and Hygiene

JF - The American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 6

ER -