The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. Copyright � 2005 by The American Society of Tropical Medicine and Hygiene.
|Number of pages||3|
|Journal||American Journal of Tropical Medicine and Hygiene|
|Publication status||Published - 2005|
Gal, D., Mayo, M., Spencer, E., CHENG, A., & Currie, B. (2005). Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. American Journal of Tropical Medicine and Hygiene, 73(6), 1162-1164.