@article{031d6db93d0a458080d5344540ba937b,
title = "Simultaneous determination of multiple amino acids in plasma in critical illness by high performance liquid chromatography with ultraviolet and fluorescence detection",
abstract = "There is increasing recognition that the host response to critical illness includes derangement of multiple amino acid pathways, including amino acids (AAs) central to metabolism and immune, endothelial and neurological function. To characterise concentration changes of these plasma amino acid we report the development and validation of a method for the quantification of AAs in small volumes of plasma (50. ?L) using HPLC with simultaneous UV and fluorescence (FL) detection. Protein precipitation and pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) is followed by reversed phase HPLC separation. Calibration curves were built with norleucine as an internal standard. Thirty-three (including the 20 proteinogenic) AAs, were selected as standards and their corresponding concentrations in the plasma of healthy human controls and patients with severe falciparum malaria were quantified. This method enables the detection of perturbations in arginine metabolism, aromatic amino acid pathways, methionine transsulfuration and transmethylation pathways and other metabolic pathways. � 2013 Elsevier B.V.",
keywords = "6-aminoquinolyl-N-hydroxysuccinimidyl carbamates, Critical illness, Fluorescence detection, HPLC, Malaria, Precolumn derivatizations, Protein precipitation, Simultaneous determinations, High performance liquid chromatography, Metabolism, Physiology, Plasmas, Precipitation (chemical), Amino acids, 1 methyl histidine, 4 hydroxy proline, 6 aminoquinolyl n hydroxysuccinimidyl carbamate, amino acid, arginine, asparagine, aspartic acid, citrulline, cystathionine, cystine, diaminobutyric acid, glutamic acid, glutamine, glycine, homoarginine, kynurenine, leucine, methionine, norleucine, ornithine, phenylalanine, serine, taurine, threonine, tryptophan, tyrosine, unclassified drug, amino acid analysis, amino acid blood level, article, controlled study, critical illness, fluorescence, human, human tissue, limit of detection, limit of quantitation, malaria falciparum, precipitation, priority journal, reversed phase high performance liquid chromatography, FL, Adult, Amino Acids, Chromatography, High Pressure Liquid, Critical Illness, Humans, Malaria, Falciparum, Reproducibility of Results, Sensitivity and Specificity, Sepsis, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet",
author = "Hao Wang and Yvette McNeil and Tsin Yeo and Nicholas Anstey",
year = "2013",
doi = "10.1016/j.jchromb.2013.09.016",
language = "English",
volume = "940",
pages = "53--58",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
}