Abstract
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-associated infection, but there is growing awareness of the emergence of multidrug-resistant lineages in community settings around the world. One such lineage is ST772-MRSA-V, which has disseminated globally and is increasingly prevalent in India. Here, we present the complete genome sequence of DAR4145, a strain of the ST772-MRSA-V lineage from India, and investigate its genomic characteristics in regards to antibiotic resistance and virulence factors.
Results: Sequencing using
single-molecule real-time technology resulted in the assembly of a single
continuous chromosomal sequence, which was error-corrected, annotated and
compared to nine draft genome assemblies of ST772-MRSA-V from Australia,
Malaysia and India. We discovered numerous and redundant resistance genes
associated with mobile genetic elements (MGEs) and known core genome mutations
that explain the highly antibiotic resistant phenotype of DAR4145.
Staphylococcal toxins and superantigens, including the leukotoxin
Panton-Valentinin Leukocidin, were predominantly associated with genomic
islands and the phage ? -IND772PVL. Some of these mobile resistance and
virulence factors were variably present in other strains of the ST772-MRSA-V
lineage.
Conclusions: The genomic characteristics presented here emphasize
the contribution of MGEs to the emergence of multidrug-resistant and highly
virulent strains of community-associated MRSA. Antibiotic resistance was
further augmented by chromosomal mutations and redundancy of resistance genes.
The complete genome of DAR4145 provides a valuable resource for future
investigations into the global dissemination and phylogeography of
ST772-MRSA-V.
Original language | English |
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Pages (from-to) | 1-10 |
Number of pages | 10 |
Journal | BMC Genomics |
Volume | 16 |
Issue number | 388 |
DOIs | |
Publication status | Published - 16 May 2015 |