The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems that will be commonly used in the laboratory situation- bacterial, yeast and insect cell culture. The application of each, and the relative advantages/disadvantages are discussed.
Jayaraj, R., & Smooker, P. M. (2009). So you need a protein-A guide to the production of recombinant proteins. The Open Veterinary Science Journal, 3, 28-34. https://doi.org/10.2174/1874318809003010028