So you need a protein-A guide to the production of recombinant proteins

Rama Jayaraj, Peter M Smooker

Research output: Contribution to journalArticle

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Abstract

The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems that will be commonly used in the laboratory situation- bacterial, yeast and insect cell culture. The application of each, and the relative advantages/disadvantages are discussed.
Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalThe Open Veterinary Science Journal
Volume3
DOIs
Publication statusPublished - 2009
Externally publishedYes

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